Mitochondrial prepp
Per Bergman
Per.Bergman at vf.slu.se
Tue Nov 29 06:35:29 EST 1994
Hi out there
We are doing western-blotts on crude preparations of tobacco mitochondria
(mt). Our antibody reacts (strong enough to be a unwanted) to BSA which we
include in our mt prepp buffer. What should we do?
1) Exclude BSA from the buffer - we do not do respiratory measurments or
in organello translations at the moment, so it it needed.
2) block-out (titer-out) BSA reacting antibodies in some way?
Thank´s in advance for advice,
Per Bergman
Per.Bergman at vf.slu.se
More information about the Methods
mailing list