Mitochondrial prepp

Per Bergman Per.Bergman at vf.slu.se
Tue Nov 29 06:35:29 EST 1994


Hi out there
We are doing western-blotts on crude preparations of tobacco mitochondria
(mt). Our antibody reacts (strong enough to be a unwanted) to BSA which we
include in our mt prepp buffer. What should we do? 
1) Exclude BSA from the buffer - we do not do respiratory measurments or
in organello translations at the moment, so it it needed.
2) block-out (titer-out) BSA reacting  antibodies in some way?

   Thank´s in advance for advice,
   Per Bergman

   Per.Bergman at vf.slu.se



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