Methods November 1994 Archives by author

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Starting: Tue Nov 1 00:10:15 EST 1994
Ending: Wed Nov 30 23:42:50 EST 1994
Messages: 1248

John Taggarts address "PBS::NELSONJ" at PBS.DFO.CA
wizard dna clean up "PBS::NELSONJ" at PBS.DFO.CA
GST fusion prot.- HELP *. *
RNA storage in guanidinium isothiocyanate *.*
RNA content of single cells? *.*
Need pIBI76--no longer made
PCR-RFLP: precipitate or dilute PCR buffer
HA epitope tag problems -sc2.sdsc.edu
help wanted for L6 cell differentiation Grad Student Lab 1
PCR question Grad Student Lab 1
Transformation of Aeromonas 190802751 Lawrence, Paul
DEPC treated water in PCR Enrique Jose Labadan Frio - SCBC - 3421101
Help! low recovery yeild of geneclean Wises Namwat - SCMI - 3636183
Pichia expression 3LCID at QUCDN.QueensU.CA
BLOCKING SLN FOR DIG-SYSTEM!! 4700gbera at umbsky.cc.umb.edu
minipreps for sequencing AKravetz
urea/SDS, guanidine/SDS PAGE AKravetz
What is Resin in Magic mini-preps? AKravetz
ethanol treatment vs. plasmid loss AKravetz
TOPOGEN, INC. - ADDRESS ARCIB at BIOTECHNET.COM
LOOKING FOR TWO CO. ADDRESSES ARCIB at BIOTECHNET.COM
ADDRESS OF TWO COMPANIES ARCIB at BIOTECHNET.COM
The trouble with L cells Mr Hassan Abdulrazzak
491 Prep Cell opinions? Dale Yuzuki's Work Account
EZ-COMP kit experiences? David Adelson
UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ BIOSCI Administrator
IMPORTANT - SERVICE INTERRUPTION LAST WEEKEND BIOSCI Administrator
cycle sequencing - dimensions Birgit Aimer
Carbohydrate against DNA Mark Hicks - Genetics Group/University of Alberta
[Q] HELP ON CHOICE OF NON-RAD LABELLING/DETECTION SYSTEMS Abreu Grobois Federico Alberto-ICML
Level 2 Biosafety Allergan
Vaccinia Virus Expression Allergan
Phosphorimager Allergan
pET and ampicillin Craig A. Altier
myc ab Danny Altschuler
Source for Hygromycin B Danny Altschuler
Luciferase antibody Keith Alvares
DEPC treated water in PCR Eric C. Anderson
get rid of developer stains from clothing Eric C. Anderson
minipreps for sequencing Eric C. Anderson
Soliciting Comments on Sequence Analysis Software Eric C. Anderson
temperature sensitive mutants Eric C. Anderson
How to Calculate Oligo MW? Eric C. Anderson
gene sequencing-HELP Eric C. Anderson
HOME-MADE GENECLEAN KIT Eric C. Anderson
Which Electroporator for E.coli transformation? Eric C. Anderson
cloning with Ecl XI cut fragment. Roger F. Anderson
Address for Genentech and Genzyme Roger F. Anderson
Using Glycogen to prec DNA??? Seanna Annis
PCR from dried sequencing gel? Bruce Appel
Clontech Two-hybrid libraries. Tracy Aquilla
RNaseA activity at elevated temp.? Tracy Aquilla
advice on phosphorimager purchase Tracy Aquilla
Promega technical support Tracy Aquilla
Chemiluminescence and the PhosphorImager? Tracy Aquilla
RNA storage in guanidinium isothiocyanate Tracy Aquilla
use of spermidine to remove DNA from prot. solutions Tracy Aquilla
help Ahmet Arman
Forwarded: Returned mail: User unknown Ahmet Arman
Rf: EDTA in cell fractionatio Curt Ashendel
chilis Curt Ashendel
GST fusion protein won't elute Curt Ashendel
Rather simple ? regarding gel electrophoresis Curt Ashendel
Is there Comercial AP Endonuclease ??? Jason Aten
SS Nytran-- Please Help! Jason Aten
Antibody warning Graham Atherton
Antibody warning Graham Atherton
CD40 B cell culturing system Graham Atherton
PCR with radioactive nucleotides B.L.Cohen
REQUEST ASSISTANCE WITH IMMUNOFLUORESCENCE BARNETT.YA
tk KNOCK OUT MOUSE..IS IT POSSIBLE? BARNETT.YA
ICN translabel trouble AMRUT S BHOGLE
cDNA preparation from <500 cells Donald W. Back
PCR-PROPDUCT PURIFICATION J.J. Baker
ds DNA sequencing surefire protocol wanted. J.J. Baker
Phosphorimager T Bannor
Test-don't read T Bannor
Tritium screens / protein gels T Bannor
Phosphorimager T Bannor
advice on phosphorimager purchase T Bannor
chilis Dave Bates
vertebrate expression vectors Dave Bates
hydroxylamine mutagenesis Hinayana Bawagan
temperature sensitive mutants Hinayana Bawagan
temperature sensitive mutants Hinayana Bawagan
Pichia expression Clifford Beall
Lambda DNA resists digestion Tom Beals
HELP! Lane background in Northern's Bruno Becker
Panhandle PCR Dick Beeman
Sequencing near primer problems. Jaideep Behari
specific 'nested' priming for reverse transcription in RT-PCR Soenke Behrends
[Q] cycle sequencing kits? Bob Belas
[Q] cycle sequencing kits? Bob Belas
pET vectors Bob Belas
(none) Belikov
EMBL4N sequence ? Callum Bell
Trouble with PCR amplification of vector inserts Robert M Bellin
5' RACE questions: BRL/Clonetech kits ? Neil Bence
betagalactosidase antibodies M K Bennett
Question on chillies M K Bennett
PCR with radioactive nucleotides M K Bennett
recombinant adenovirus? Benoit_Hebert at IAF.UQUEBEC.CA
DNASIS Benoit_Hebert at IAF.UQUEBEC.CA
EDTA in cell fractionatio N Benson
Mitochondrial prepp Per Bergman
Cosmid Sequencing Advice Stephen Beverley
Help! Hexyl-CoA-Agarose B. Ganesh Bhat
Address for Genentech and Genzyme Ajay Bhatia
oligo houses Lisa Bibbs
gst elution Robert Solomon Bioc
DNA analysis programs (lack of) David F. Bishop
MSDS (or COSHH) Shaun D. Black
Info server for Primers, Probes useful in natural populations Peter T. Boag
MOLECULAR ECOLOGY POSTDOCS NEEDED Peter T. Boag
Methylation analysis/bisulphite sequencing Jeffrey H. Boatright
Schneider SL-2 cells Peta C. Bonham-Smith
SSCP-PCR & methylmercury hydroxide Dr Stuart Boyer
immunostaining COS-7 cells Jeffrey D. Bray
Spectrophotometer Help John Brookes
pET and ampicillin Dr. R.A. Brooksbank
How to Calculate Oligo MW? Nigel Brown
How to Calculate Oligo MW? Nigel Brown
T3/T7 PRIMER QUESTION!!! Stuart Brown
ABI Support software for automated sequencing Stuart Brown
help:how tomake 250ug 130bp fragment? Karen Browning
Advse needed on DNA strand-separation Karen Browning
DNA analysis programs (lack of) Karen Browning
advice on phosphorimager purchase John Brunstein
EZ-COMP kit experiences? John Brunstein
pET and ampicillin Paul A Bucciaglia
Psoralen biotin probe Jason Buerkle
bad experience with a company Andreas Buhr
Xenopus oocytes/expression/DNA injection nucleus Andreas Buhr
mRNA capping kits Andreas Buhr
help on pIBI30 Anhthu Bui
c-myc tag immunoprecipitation William Bunnell
smallest transfected transmembrane protein William Bunnell
smallest transfected transmembrane protein William Bunnell
Phosphorimager Jim Burrows
DOT BLOTS using BIOTINYLATED probes Jim Burrows
SS Nytran-- Please Help! Timothy Bushnell
MSDS software Timothy Bushnell
unable to ppt. protein using ammonium sulphate Tim Buss
Editor for cDNA sequence publication CBA0031 at uabdpo.dpo.uab.edu
Clone a unknow enzyme CBA0031 at uabdpo.dpo.uab.edu
***Example of moleculer evolution*** CBA0031 at uabdpo.dpo.uab.edu
Phosphordiesterase I Huesken Dieter MSM FO23 CH
CA repeat at the 3' end CHAUDH45 at CCVAX.MMC.EDU
digestion of genomic DNA from mouse tails Rob Campbell
Question on chillies Roy G. Cantrell
help sequence from puc Dave Carmean
Vancouver Biotech Phone #/Address Dave Carmean
Cloning with LambdaGEM-12 Fiona Cassidy
Cloning problems with LambdaGEM-12 Fiona Cassidy
Algae project, need advice please. Amy Caudy
SYBR Green Chad
hnRNP Ab Source Daniel Chagnovich
Phosphoimagers etc revisited Daniel Chagnovich
Phosphoimagers etc revisited Daniel Chagnovich
Could we direct PCR of human hair shafts? Wasun Chantratita
Need help for C.trachomatis momp1 gene sequence Wasun Chantratita
PCR David Char
how to make sigle-stranded DNA of pET Yee-Yung Charng
labeled LDL? Devasis Chatterjee
LA PCR info ?!? Mic Chaudoir
Loading Long Ranger gels... Mic Chaudoir
Soliciting Comments on Sequence Analysis Software Mic Chaudoir
How to Calculate Oligo MW? Mic Chaudoir
Media Formula Question Mic Chaudoir
DNA analysis programs (lack of) Mic Chaudoir
Which Electroporator for E.coli transformation? Mic Chaudoir
What is the best nylon membrane? Mic Chaudoir
cDNA library of Hen Oviduct - Needed Chemotaxis
--- Chicken oviduct cDNA NEEDED --- Chemotaxis
Alkaline phosphatase? Jeremy Chen
Sequencing Gel Fixation Tsute Chen
DNAse contamination Dr. Chihara
5' RACE Specificity Problems Sarah Childs
test Nikolai Chitaev
Test Nikolai Chitaev
sequence comparison Giltsu Choi
Cloning with LambdaGEM-12 Chutney
microsatellite help Claire.Billot
Photodocumentation Duncan Clark
"LONG" RT/PCR Duncan Clark
Long & Accurate PCR Duncan Clark
Perplexing result with site-directed mutagensis Duncan Clark
PCR: Why is my TAQ unfaithful Duncan Clark
superoxide dismutase Evelyn Clausnitzer
Chomczynski traumas! Michael Coady
DNASIS Randall J. Cohrs
help sequence from puc Randall J. Cohrs
Chomczynski traumas! Randall J. Cohrs
theory and acetate Randall J. Cohrs
Human codon bias Randall J. Cohrs
?best purification of 186bp DNA fragment from agaraose Robert C. Colgrove
sample prep for ATP determination James T. Colston
Mouse Testis Two Hybrid Library Dr. H.J. Cooke
Homemade GeneClean kits ??? Michael Cooley
Odd problem with RAPD sequencing Michael Cooley
Use of Amersham's Hybond N+ Michael Cooley
Use of Amersham's Hybond N+ Michael Cooley
What is Resin in Magic mini-preps? Michael Cooley
Using Glycogen to prec DNA??? Michael Cooley
Has anyone cloned a RAPD band? Michael Cooley
DNAse contamination in plasmid preps Michael Cooley
PCR from dried sequencing gel? Michael Cooley
Cleaning Electroporation Cuvettes Michael Cooley
Direct cloning of a bacterial gene Michael Cooley
wizard dna clean up Michael Cooley
cDNA preparation from <500 cells Michael Cooley
oligo hybridization John Cooper
oligo purification on hplc John Cooper
Sequencing Gel Fixation John Cooper
ES cells Seth Corey
HELP-Optimization of B-gal Carrie Elizabeth Couper
Mucopolysaccharide staining David T. Croke
Platinum/Biotin complex Hugues Roest Crollius
DuPont's new autorad film Van Wayne Crouch
Help with biotin-labeled DNA probes CrouseJ
Stephen Jay Gould The PCR Cruncher
Techne Gene E thermocyclers Dr. T.E. Cullingford
E. coli gene probes? DAILEYD at LYNX.APSU.EDU
Question on chillies DE ANGELIS,DINO
HA epitope TAG: is 12CA5 the only Ab available? DE ANGELIS,DINO
requesting address for BABCO and Southern Biotechnologies DE ANGELIS,DINO
electrophoresis of nuclear extracts DNewmeyer
Xenopus oocytes/expression/DNA injection nucleus DNewmeyer
DSM-email ? Slawek Dabrowski
Lambda-offert Slawek Dabrowski
Vectors of the Past? Miss. E.C. Dalton
in situ PCR Miss. E.C. Dalton
Easy/CHEAP Genomic DNA Richard Dana
Algae project, need advice please. Richard Dana
phage prep help really, really needed Joshua Daniels
Borate inhibition of T4 DNA ligase Darren.Martin
Circular DNA and Stables David J. Austin, Ph.D.
Use of Amersham's Hybond N+ David L. Haviland, Ph.D.
Promega technical support David L. Haviland, Ph.D.
immunostaining COS-7 cells Nancy David
tagging membrane proteins Nancy David
requesting address for BABCO and Southern Biotechnologies Nancy David
promoter for transgenics? Nancy David
ISOLATION OF RNA FROM PLANTS - METHODOLOGY QUESTION Ed Davis
ISOLATION OF RNA FROM PLANTS - METHODOLOGY QUESTION Ed Davis
Homemade GeneClean kits ??? Simon Dawson
Homemade GeneClean kits ??? Simon Dawson
MSDS (or COSHH) Simon Dawson
Chomczynski traumas! Simon Dawson
Using Glycogen to prec DNA??? Simon Dawson
Soliciting Comments on Sequence Analysis Software Jim Decker
fingerprint DNA Dieter Deforce
ABI 373 DNA sequencer Dieter Deforce
Help! Dye terminator sequencing Dieter Deforce
UV-light for DNA linking and destruction Dieter Deforce
Could we direct PCR of human hair shafts? Dieter Deforce
DNA Strider: Where can I get it? Michael Deyholos
Source for poly(ribothymidine) aka poly(5-methyluridine) Dan Diaz
ethanol treatment vs. plasmid loss Luis Diaz
*** Q: WHAT KIND OF PEOPLE ON THE NET ? Pierre Didierjean
Please send me the BIOSCI FAQ. Thanks Ruediger Dietrich
BIOSCI FAQ Ruediger Dietrich
Chomczynski traumas! John Dixon
lambda packaging kits John Dixon
epithelial cell culture John Dixon
Genomic DNA from E.coli without phenol or chloroform?? Gabriel Dorado
AUSTRALIA, Sidney, St. Vincent's Hospital, Dr. J.A. Eisman et al?? Gabriel Dorado
Genomic DNA from E.coli without phenol or chloroform?? Gabriel Dorado
AUSTRALIA, Sidney, St. Vincent's Hospital, Dr. J.A. Eisman et al?? Gabriel Dorado
AUSTRALIA, Sidney, St. Vincent's Hospital, Dr. J.A. Eisman et al?? Gabriel Dorado
Genomic DNA from E.coli without phenol or chloroform?? Gabriel Dorado
AUSTRALIA, Sidney, St. Vincent's Hospital, Dr. J.A. Eisman et al?? Gabriel Dorado
Genomic DNA from E.coli without phenol or chloroform?? Gabriel Dorado
373A Wizard Miniprep (Promega) for 373A ABI Sequencer Gabriel Dorado
373 Wizard Miniprep (Promega) for 373A ABI Sequencer Gabriel Dorado
RT-PCR (PRIMER DESIGN FOR RACE) Gabriel Dorado
Looking for Michael A. Frohman (RACE) Gabriel Dorado
Help on pIBI30 Marcelo C. Dornelas
Jonathan please contact Dorothea Dorothea.Smith
stabilty of biotinylated primers Douglas
Is DGGE the best? Dimitrios Doumas
Primers for DGGE? Dimitrios Doumas
Vector sequences? Mr TG Downing
Promega technical support Ken Doyle
beta-galactosidase Mike Dyall-Smith
Address of BBL microbiology Systems Martineau P. E.
Question on chillies Nigel Eastmond
Photodocumentation Nigel Eastmond
Chemiluminescence and the PhosphorImager? Nigel Eastmond
Interleukin-1B cDNA Sequence Nigel Eastmond
Quantitative protein determinations in plant extracts Ekramoddoullah, Abul Kalam M.
DNAStar Program(s) Bruce Elder
Liver cDNA library(ies) Bruce Elder
(none) Cheah Kathryn Song Eng
Protean II glass breaking Cohen Eric
AminoAcid sequence of Bovine MBP Mr J.S. Erikson
G3PD +ve control primers Mr J.S. Erikson
RNA isolation from PANCREAS!!! Mr J.S. Erikson
TAP (Tobacco Acid Pyrophosphatase) wanted Elizabeth Estabrook
TAP (Tobacco Acid Pyrophosphatase) wanted Elizabeth Estabrook
"LONG" RT/PCR Elizabeth Estabrook
Long & Accurate PCR Elizabeth Estabrook
PCR crash: Feedback! PANIAGUA SOLIS JORGE FERNANDO
Pwo PCR: info wanted PANIAGUA SOLIS JORGE FERNANDO
oligo antisense Juliette Faraco
non-radioactive RNAse protection assays Patrice Ferriola
Who sells myc monoclonal Ab? H. David Fischer
how to make sigle-stranded DNA of pET Karl Fischer
GST -c-jun Seth M Fisher
Alternatives to MYC epitope tag Seth M Fisher
FLAGged Protein Purification Seth M Fisher
calculating extinction c Spencer Fisk
In-Situ PCR T.J. Fitzmaurice
Quantitative protein determinations in plant extracts Ragnar Flengsrud
SYBR Green I Filter Parke K. Flick
Cleaning Electroporation Cuvettes Ian James Forsythe
Single lane/column sequencing-help John E. Fox
RECOMMENDATION FOR FREEZE DRYERS John E. Fox
pcr amplification from paraffin-embedded tissue sections Tom Frank
PCR Tom Frank
FIAU Ahnders Franzen
FIAU Ahnders Franzen
Tissue specific promotors Ahnders Franzen
FIAU Ahnders Franzen
FIAU Ahnders Franzen
FIAU again ! Ahnders Franzen
Lightsmith II Sherri Fraser
Seeking EGF for use as a wound-healing drug Marion Freistadt
mRNA capping kits Otto Froehlich
gelatin filters for EtBr photography FrohlichM
help:how tomake 250ug 130bp fragment? G
Genomic DNA from E.coli without phenol or chloroform?? G
Advse needed on DNA strand-separation G
Luciferase antibody G.E.Fazzi
CYCLO OXYGENASE antibody commerially available? G.E.Fazzi
sonication+GTC method get better RNA GEORGE XIAOXI OUYANG, ELECTRICAL ENGINEERING, PHONE 579-0970
Ampicillin inactivation temp. GWDGV1::KCHOWDH
Req: Definition of a promoter showing tissue specificity GWDGV1::KCHOWDH
Req: Definition of a promoter showing tissue specificity GWDGV1::KCHOWDH
Req: Definition of a promoter showing tissue specificity GWDGV1::KCHOWDH
Asksam? Frederick Garbrecht
stabilty of biotinylated primers Frederick Garbrecht
Antibody warning Frederick Garbrecht
Mutation detection Gareth
pJAY3 Vector Stephen Gately
5'-end labeling of 8mer oligo? Peter Gegenheimer
Use of Amersham's Hybond N+ Charlie Wright Genetics
best way to remove PCR primers Charlie Wright Genetics
Theoretical Melting Temperature of PCR primers? Charlie Wright Genetics
PCR-PROPDUCT PURIFICATION Charlie Wright Genetics
Re-bulkprep problems Michael Gieseg
HELP! Lane background in Northern's Gil
HELP! Lane background in Northern's Gil
Phage DNA preparation Steven Goldberg
plasmid prep with G+ bacteria Steven Goldberg
Plasmid Deletions - Exo or DNAse? Marc Goldstein
Activated gel supports. Comments? Marc Goldstein
PCR: Why is my TAQ unfaithful Marc Goldstein
Genbank comparison Marc Goldstein
RNases A in sol. 1 Liam E Good
Southern blot nightmare Anita Gould
gelatin filters Anita Gould
Pichia expression Kathryn Graham
Smallest selectable eukaryotic marker? Roger Graham
Server to predict leader sequences? Roger Graham
GC Contents? Catharine Gunson
WGA-Agarose column? Xin Guo
Synthetizing probes with Taq Guy.Mordret
(none) H0C2028 at ACS.TAMU.EDU
Odd problem with RAPD HARDIES at THORIN.UTHSCSA.EDU
lambda DNA resists digestion HARDIES at THORIN.UTHSCSA.EDU
primer-dimers in pcr HARDIES at THORIN.UTHSCSA.EDU
Do you need supercoiled pDNA for ds-seq.? HARDIES at THORIN.UTHSCSA.EDU
PCR primer design (keep eye on 3' end) HARDIES at THORIN.UTHSCSA.EDU
EcoRI contaminated (so use less) HARDIES at THORIN.UTHSCSA.EDU
What is Resin in Magic mini-preps? HEAZLEWOOD,Joshua
Types of Resin in Magic(Merlin) Minipreps HEAZLEWOOD,Joshua
source of FIAU HRMBDKC at HKUMD1.HKU.HK
FIAU again HRMBDKC at HKUMD1.HKU.HK
TAP (Tobacco Acid Pyrophosphatase) wanted Yoonsoo Hahn
mRNA capping kits Alan Hair
nylon nets for hybaid tubes A.J.W. Hall
Need Help with Northern Blot Brian Halloran
DNA from paraffin-tissue? Brian Halloran
RNA storage in guanidinium isothiocyanate Andre Hamel
yeast contamination R Hanemaaijer
Texas Red labeling of antibodies Richard R. Hardy
Soliciting Comments on Sequence Analysis Software Richard R. Hardy
Panhandle PCR Bob Harrison
Nightmare of Baculovirus expression system Jane E B Harrison
T4 DNA Polymerase-Buffer Conditions Yasha Hartberg
Sequencing Gel Fixation Yasha Hartberg
plasmid curing Takashi Hashimoto
curing plasmids Takashi Hashimoto
e-mail address Takashi Hashimoto
Human chromosome-specific genomic libraries Randy Haun
SmaI/XmaI digests Philip Heacock
calculating extinction coefficient from protein sequence ??? Marcus Heller
5'-end labeling of 8mer oligo? Barry Henderson
Prep remains of mummies? Paul N Hengen
Frequently Asked Question (FAQ) list Paul N Hengen
chemiluminescent detection Paul N Hengen
registered trademark insignia Paul N Hengen
New DNA stain Paul N Hengen
PCR with radioactive nucleotides Paul N Hengen
What is spermidine??? Paul N Hengen
chemiluminescent detection Paul N Hengen
best way to remove PCR primers Paul N Hengen
Primer stocks that fail to PCR over time Paul N Hengen
dsDNA sequencing protocol Paul N Hengen
dsDNA sequencing protocol Paul N Hengen
PRIMER-DIMERS in PCR Your Name Here
RNA storage in guanidinium isothiocyanate Robert Hewitt
cDNA preparation from <500 cells Robert Hewitt
What is Resin in Magic mini-preps? Bernard Heymann
Nightmare with GST fusion protein Bernard Heymann
Carbohydrate against DNA Thomas Hiesberger
Carbohydrate against DNA summary Thomas Hiesberger
32P into Supercoiled -No way ??? Thomas Hiesberger
PROTEIN PURIFICATION WOES - AN UPDATE!!! Andy Hill
PCR with radioactive nucleotides Timo Hiltunen
G3PD +ve control primers Timo Hiltunen
PCR primer mismatches Timo Hiltunen
New DNA stain Robert Hindges
TECHNE thermal cyclers Tony Hodge
a problem in protein purification D. Hodges
Dumb question (?) re cycle sequencing of PCR products Guy Hoelzer
PCR with radioactive nucleotides Annette C. Hollmann
Anti-ser/thr phosphorylated proteins Gene Holowachuk
RNA from archival/fixed tissue Steve Hopkins
Techne Gene E thermocyclers Steve Hopkins
TGFbeta2 Kathleen Hopp
outage? Kathleen Hopp
PCR product stability Robert Horton
in vitro transcription reactions Randall Howard
Advise needed on DNA strand-separation Baohua Huang
Perplexing result with site-directed mutagensis I-Jen Huang
chilis David S. Huen
RNaseA activity at elevated temp.? Olav Hungnes
O.C.T.? Jim Hutchins
>>>>>Help on Hybridization Tm<<<<< CJF 90/10 INSERM
cloning with Ecl XI cut fragment. Kenneth Y. Ilio
recombinant adenovirus? J.C. Irminger
Test J.C. Irminger
test J.C. Irminger
A recipe for whole-mount in situ hybridization on vertebrate embryos David Ish-Horowicz
A recipe for whole-mount in situ hybridization on vertebrate embryos David Ish-Horowicz
A recipe for whole-mount in situ hybridization on vertebrate embryos David Ish-Horowicz
New DNA stain Masaaki Iwabuchi
UV Source for SYBR GREEN? J.Carnwath
Rather simple ? regarding gel electrophoresis J.Chang
Western blot-polysomes problem JMORELLI at MAINE.MAINE.EDU
DNAgency Oligos Jack M. Bernstein, M.D.
PROMOTER BANK Jack M. Bernstein, M.D.
Clogged G-amidite lines Jack M. Bernstein, M.D.
How to Calculate Oligo MW? robert w. Jackman
how to make sigle-stranded DNA of pET Don Jackson
Shotgun sequencing assembly Richard James
Flagging or hexaHis tag? Richard James
hydroxylamine mutagenesis Richard James
ALF DNA Sequencer Discussions Richard James
pET and ampicillin Richard James
discrepancies between cDNA and protein sequences Le Pennec Jean-Paul
re thanks for answering Le Pennec Jean-Paul
chilis Pierre Jelenc
help purification of 30kb plasmid JoeJoe
Direct sequencing of lambda phage dna David Johnson
Nuclear Factor-DNA Binding Motifs David Johnson
Phage DNA Todd Johnson
Dumb question (?) re cycle sequencing of PCR products (Dave Johnston)
b-gal in Xenopus Jillian Johnston
Problems in cloning with LambdaGEM-12 Meirion Jones
HELP! Problems with the expression of HSP 70. Zophonias O. Jonsson
Genbank comparison Zophonias O. Jonsson
ribo- or RPL probe ? Malcolm Moos Jr.
ribo- or RPL probe ? Malcolm Moos Jr.
Panhandle PCR Malcolm Moos Jr.
Panhandle PCR Malcolm Moos Jr.
ssDNA of M13 methylated? MARCEL KELLER
e-mail subscription JOHN A. KORTE
(none) KSP5165
Mouse chromosomal location blots--NEEDED KT
Promoter Databases: How to search for binding-motifs?? Stefan Kahlert
RNA extraction and homogenization Fredrik Kamme
32P into Supercoiled -No way ??? Karen
Clogged G-amidite lines Karen
CAT assays (in cell culture media) J. Kaufmann
Alternatives to MYC epitope tag J. Kaufmann
Genomic DNA from E.coli without phenol or chloroform?? Michael D Kawalek
DNASIS Michael D Kawalek
T3/T7 PRIMER QUESTION!!! Martin Kennedy
<None> Martin Kennedy
FIAU again Martin Kennedy
A recipe for whole-mount in situ hybridization on vertebrate embryos In C. Kim
Need large, intact human genomic clones? Ung-Jin Kim
need info about microwave restriction digest Thorsten Kirsch
Re.: Help! PCR Problems Mike Kitamura
32P into Supercoiled -No way ??? Paul Kitts
EMBL4N sequence ? Paul Kitts
DEPC treated water in PCR Klaus.Matthaei at ANU.EDU.AU
digestion of genomic DNA from mouse tails Klaus.Matthaei at ANU.EDU.AU
How much DNA is in a single rat neuron???????? Klaus.Matthaei at ANU.EDU.AU
PCR with radioactive nucleotides Klaus.Matthaei at ANU.EDU.AU
Question on chillies Dorothy Klein
Lac Operon Mutants Ann Kleinschmidt
Antibodies to Fish snRNPs Ann Kleinschmidt
Alternatives to MYC epitope tag Susan Koester
"LONG" RT/PCR Eric Kofoid
Cleaning Electroporation Cuvettes Eric Kofoid
chilis Steve Konings
chilis Steve Konings
chilis Steve Konings
Plasmid Deletions - Exo or DNAse? Paul Edward Kowalski
loading long ranger gels Sasha Kraev
Dumb question (?) re cycle sequencing of PCR products Sasha Kraev
Plasmid sequencing Sasha Kraev
pea cDNA/B.napus Southern problem Sasha Kraev
Southern nightmare problem Sasha Kraev
Silver sequencing Sasha Kraev
RAPD cloning Sasha Kraev
sequencing gel fixation Sasha Kraev
Nylon membrane blues Sasha Kraev
Southern anti-nightmare Sasha Kraev
Filters for EtBr photography Sasha Kraev
Filters for EtBr photography Sasha Kraev
chemiluminescent detection Sasha Kraev
More on detecting chemiluminescence Sasha Kraev
Large-ish vectors that won't behave Sasha Kraev
Cycle sequencing large inserts Sasha Kraev
dsDNA sequencing protocol Sasha Kraev
GST fusion protein won't elute Cornelius Krasel
discrepancies between cDNA and protein sequences Cornelius Krasel
Genbank comparison Cornelius Krasel
Protean II glass breaking Cornelius Krasel
Primer Extension Cornelius Krasel
dsDNA sequencing protocol Cornelius Krasel
Drug to kill actively growing yeast cells Tony Kwan
UV-light for DNA linking and destruction M O L B I O L
test do not read martin LEACH
milky PAGE gel-what gives?? LKM100F at ODUVM.BITNET
Qiagen columns LMS
Source for HA antibody in Europe? LOGAN
Rf: EDTA in cell fractionatio LOGAND
Rf: Subcellular Fractionation LOGAND
separation ss from annealed ds-oligos LONSDALE
cheap oligos Hanson Lab
unable to ppt. protein using ammonium sulphate S.A. Benson Lab
cDNA RDA SchatzD2 Lab
HA epitope tag problems Tsichlis Lab
Protein:RNA crosslinking Michael Robert Ladomery
Has anyone cloned a RAPD band? Mark C Lagace
Small scale RNA /DNA preps Elise Lamar
Lambda DNA resists digestion Carlisle Landel
Chomczynski traumas! Carlisle Landel
PCR with radioactive nucleotides Carlisle Landel
PCR-PROPDUCT PURIFICATION Carlisle Landel
RNA-Isolation from plant seeds using CTAB J. Lang
Do you really relax with Hoefer SixPac ? Markus Laub
DNAStar Program(s) Carl Lawyer
Promoter Databases: How to search for binding-motifs?? Carl Lawyer
Soliciting Comments on Sequence Analysis Software Carl Lawyer
DNAStar Program(s) Carl Lawyer
Who sells myc monoclonal Ab? Martin Leach
E-mail of Murray J. Cairns or Vincent Murray (Univ of N.S.W. AU) Martin Leach
CAT assays (in cell culture media) Martin Leach
Req: Definition of a promoter showing tissue specificity Martin Leach
nylon nets for hybaid tubes Martin Leach
primary rat hepatocytes.. Martin Leach
Clogged G-amidite lines Martin Leach
luciferase detection Martin Leach
HELP!! Artificial poly(A)!! JongSub Lee
In situ PCR of Plant tissue JongSub Lee
Need help for C.trachomatis momp1 gene sequence Pranee Leechanachai
Need help for C.trachomatis momp1 gene sequence Pranee Leechanachai
testing, please ignore Pranee Leechanachai
HA epitope tag problems K. Legate
Antheprot-urgent Rainer Lemke
What is EDF film ? Gang Li
GenHunter Jonas Lindeberg
HELP! Lane background in Northern's Lauri Lintott
best purification of 186bp DNA fragment from agarose Lauri Lintott
problems in purification of small DNA fragments Lauri Lintott
Cloning with LambdaGEM-12 Eric Lippay
Oligo Labeling With Terminal Transferase Eric Lippay
Sequencing with degenerate primers Eric Lippay
What is Resin in Magic mini-preps? Mark Jason Logan
Loading Long Ranger gels... John W. Longshore
T3/T7 PRIMER QUESTION!!! John W. Longshore
Electroporators: what brand? Heather H Lord
Oxygene - Dallas??? Heather H Lord
RECOMMENDATION FOR FREEZE DRYERS Heather H Lord
pCG4 plasmid availability Patric Lundberg
Reference/advice for in situ PCR? Alan Lyons
help with laarge plasmids M.A.Schmidt
help with commercial source of transducin M.A.Schmidt
In vitro translation kit? BARRY J MARGULIES
Regulatory seq. database MBDMITRY at CCVAX.SINICA.EDU.TW
electrophoresis of nuclear extracts MC_CARTHY,DAVE
Purine Rich Region in Promoter DAVID MITCHELL
Sizes of rRNA MUNGO at UVVM.UVIC.CA
Alternatives to MYC epitope tag NEAL MUSTO
Globomycin Jixiang Ma
Use of Amersham's Hybond N+ Emma Macfarlane
Qiagen columns MahGuh
dead glycerol stocks? MahGuh
urea/SDS, guanidine/SDS PAGE Malcolm Moos Jr., M.D., Ph.D.
Tn10 derivatives Stanley Maloy
pcr amplification from paraffin-embedded tissue sections Terese Maltzman
Help:companies w/ inexpensive gt10 primers? Mandalaparthy
need fax/toll free ph number of DNagency. Mandalaparthy
help w/ ecor1 contam! Mandalaparthy
Despo for gt10 primers:can any1 spare Mandalaparthy
desperate:gt10 primers-can any1 spare? Mandalaparthy
What is spermidine??? Mandalaparthy
need help w/ phage DNA digestion! Mandalaparthy
Antibodies to Thioredoxin Richard Mandel
Genbank comparison Lol Maret
Smallest Cloning Vector and Selectable Marker ... Ferenc Marincs
Olomoucine, a cdc2 Protein Kinase inhibitor? Bernard Maro
ABI 373 DNA sequencer Steve Marsh
Vancouver Biotech Richard Kenneth Bruce Martel
wizard DNA clean up Richard Kenneth Bruce Martel
DNASIS Daoust Martin
RECHERCHE LABO POUR UNE THESE : BIOLOGIE/BIOCHIMIEn Daoust Martin
Supplier for SV40 T- antigen Marc J. Mass
Nightmare with GST fusion protein Jean-Yves Masson
Smallest Cloning Vector and Selectable Marker ... Viraj Master
Smallest Cloning Vector and Selectable Marker ... Viraj Master
Photodocumentation Viraj Master
hippocampal promoter? Jeffrey N Masters
Is the HSV-1 _tk_ gene casette commercially available? Michiaki Masuda
ds DNA sequencing surefire protocol wanted. David Matthews
Lambda DNA resists digestion Rob Mauk
rho termination suppression=suppression by tRNA? Russell A. Maurer
ABI Support software for automated sequencing George Mayhew
Luciferase Problems? Steve McKinney
luciferase mutants and help Steve McKinney
Altered Sites II Mutagenesis Michael J. McLeish
Silver Sqeuencing Daniel Mcgoldrick
RNases A in sol. 1 Jim Meador
Help! PCR Problems Yang Hsiang Mei
SS Nytran-- Please Help! Daniel Meier
What is the best nylon membrane? Daniel Meier
Fluorogenic Beta-gal substrates William Meikrantz
Use of Amersham's Hybond N+ Grace Mendoza
Substrates for B-GUS and B-GAL ? Eric H. Mercer
Req: Definition of a promoter showing tissue specificity Eric H. Mercer
Unusual 3' RACE artifact? David J. Meyer
PCR: Why is my TAQ unfaithful David J. Meyer
a problem in protein purification David Micklem
Using Glycogen to prec DNA??? David Micklem
Neuronal cell line with glutamate receptors?? Steve Miller
HA epitope TAG: is 12CA5 the only Ab available? Steve Miller
Alternatives to MYC epitope tag Steve Miller
Which Electroporator for E.coli transformation? Steve Minchin
a problem in protein purification Ding Ming
a problem in protein purification Ding Ming
Human codon bias Ding Ming
unable to ppt. protein using ammonium sulphate Ding Ming
Physical mapping - need alignment software Robert E Minto
Packaging cell-lines for virus Laura K. Moen
Degenerate primers in PCR - Help! Michael Morales
Advice on Film Recorders Kevin Morano
Server to predict leader sequences? Kevin Morano
Request for genomic bank! Jose Moreira
ultracentrifugation MorganDonaghy
CA repeat at the 3' end Shahram Mori
Heparin in PCR? Shahram Mori
ethanol treatment vs. plasmid loss Shahram Mori
cheap oligos Shahram Mori
'Good' Buffer with pKa of 5.3-5.4? Shahram Mori
Help! PCR Problems Shahram Mori
PCR amplification of up to 30-Kb DNA Shahram Mori
GST fusion prot.- HELP Shahram Mori
Dumb question (?) re cycle sequencing of PCR products Shahram Mori
phage prep help really, really needed Shahram Mori
need fax/toll free ph number of DNagency. Shahram Mori
PCR/single 3' mismatch Shahram Mori
PCR question Shahram Mori
help w/ ecor1 contam! Shahram Mori
The Case of the Vanishing Intron Shahram Mori
Help! Western problems! Shahram Mori
! ! ! ! ! H E L P ! ! ! ! ! Shahram Mori
"LONG" RT/PCR Shahram Mori
Vent PCR conditions Shahram Mori
PCRing 5' UTR using a cDNA plasmid? Shahram Mori
5' RACE Specificity Problems Shahram Mori
plasmid curing Shahram Mori
separation ss from annealed ds-oligos Scott B. Mulrooney
! ! ! ! ! H E L P ! ! ! ! ! Graeme Mulvaney
Do you need supercoiled pDNA for ds-sequencing? Pete Muriana
help w/ ecor1 contam! Pete Muriana
32P into Supercoiled -No way ??? Pete Muriana
Purifiying insoluble protein Bernard Murray
How to Calculate Oligo MW? Peter Myler
Rather simple ? regarding gel electrophoresis Daniel Mytelka
E-Mail of Dr. Eugen W. Nester NAGEL at FRCU.EUN.EG
Optimum Mg concentration for Taq ligase & Taq polymerase Yasuhiro NAITO
Purification of cosmid DNA Mr. O.Coutelle NIMR
tissue specific gene expression NISSON at BIOTECHNET.COM
DNAgency Oligos Jon Nakamoto
need fax/toll free ph number of DNagency. Jon Nakamoto
Mussel tissue needed John J Naleway
best way to remove PCR primers Peter J. Neame
Sequencing with degenerate primers Peter J. Neame
How to Calculate Oligo MW? Richard Near
Sp1 protein source David Troy Nelson
Looking for a dog or cat placental cell line. Any suggestions? John Nelson
oligo purification on hplc Robert Nelson
Problems with DIG Katherine Nesbitt
Lambda DNA resists digestion Thomas Newman
373 Wizard Miniprep (Promega) for 373A ABI Sequencer Thomas Newman
Soliciting Comments on Sequence Analysis Software John Ngsee
GST fusion prot.- HELP Rae Nishi
chilis Rae Nishi
phage prep help really, really needed Rae Nishi
Chalfie's phone number?? re: the aequorin plasmid Rae Nishi
C-terminal HIS tag/GST/myc fusion proteins Rae Nishi
help with laarge plasmids Prenom Nom
SmaI/XmaI digests Dr. Pamela Norton
Linear vs. Circular DNA Dr. Pamela Norton
Shotgun sequencing assembly Andy Law (Big Nose)
minipreps for sequencing Andy Law Big Nose
DNAStar Program(s) Andy Law Big Nose
The Case of the Vanishing Intron Thierry Nouspikel
The Case of the Vanishing Intron Thierry Nouspikel
Long & Accurate PCR Thierry Nouspikel
Wash of GF-filters Frank Bech Nygaard
RAPD Analysis on the ABD 373 Catherine O'Connell
Sequencing near primer problems. OVan-Ham
Chalfie's phone number?? re: the aequorin plasmid Pat O_Neal
Chalfie's phone number re: the green fluor. protein plasmid Pat O_Neal
DuPont's new autorad film Denise Odonnell
Regulatory seq. database Dan Oerther
PCR primer design with RE sites Dan Oerther
What is 'gel mobility shift assay'? Dan Oerther
Direct sequencing of lambda phage dna Guilherme Co. Oliveira
Protean II glass breaking Guilherme Co. Oliveira
biotin-lys-tRNA Tetsuya Ookura
LEXA Antibodies Guy Oshiro
Were we can buy cheapest DNA primers? Torben Osterlund
ABI 373 DNA sequencer Torben Osterlund
calculating extinction coefficient from protein sequence ??? Tanja Oswald
Alu sequences? Francis Ouellette
Vector sequences? Francis Ouellette
Do you need supercoiled pDNA for ds-sequencing? Jim Owens
Do you need supercoiled pDNA for ds-sequencing? Jim Owens
gene sequencing-HELP Jim Owens
The Case of the Vanishing Intron Jim Owens
Trademark and registered TM Jim Owens
need help w/ phage DNA digestion! Jim Owens
unable to ppt. protein using ammonium sulphate Jim Owens
best way to remove PCR primers Jim Owens
RNA content of single cells? Jim Owens
Techne Gene E thermocyclers Jim Owens
PCR product stability Bengt Oxelman
regulation of tet promoter on pBR322??? PRAMATHESH PATEL
Purifiying insoluble protein ANDY PHILLIPS
test RICHARD P. PHIPPS
HA epitope tag problems PJOB
Human codon bias PJOB
Human codon bias PJOB
Drug to kill actively growing yeast cells AL R. PLUMMER
Level 2 Biosafety PMacklon
Help! PCR Problems POPOVA
"LONG" RT/PCR POPOVA
Sma I digest POPOVA
yeast contamination POPOVA
hydroxylamine mutagenesis Anthony Palombella
Help locate FAX Number/email Alain Sarasin Leon Parker
Human cell line with chromosomal, inducible lacZ Leon Parker
Human cell line with inducible lacZ gene Leon Parker
gelatin filters for EtBr photography Dave Pataky
L. lactis Northerns Christopher Coward Path
Mutation detection Patrick_Kleyn at MSMAIL.MPI.COM
PCR/single 3' mismatch Garth I. Patterson
oligo purification on hplc David J. Perry
phage prep help really, really needed Clemens Peterbauer
PCR Quantification Joe Peters
Loading Long Ranger gels... Petur Henry Petersen
Loading Long Ranger gels... Petur Henry Petersen
3' murine TCR V-beta region probe Howard T. Petrie
Sterilization a viscous medium Thanit Pewnim
pET and ampicillin Todd Miller - Pharmacology
Where Can I Buy A Hybridization Oven? Dave Pilgrim
GST fusion protein won't elute T. S. Pillay
Chemiluminescence and the PhosphorImager? T. S. Pillay
Nightmare with GST fusion protein T. S. Pillay
Plant Flow Cytometry Alfonso J. Garcia Pineres
nuclear plant DNA isolation Alfonso J. Garcia Pinesres
nuclear plant DNA extraction Alfonso J. Garcia Pinesres
help:how tomake 250ug 130bp fragment? David W. Plank
Clontech Two-hybrid libraries. Simon E Plyte
Purine Rich Region in Promoter Mike Powell
32P into Supercoiled -No way ??? Mike Powell
Soliciting Comments on Sequence Analysis Software Robert Preston
temperature sensitive mutants Robert Preston
DNAse or nuclease assay Robert Preston
milky PAGE gel-what gives?? Robert Preston
pH management Robert Preston
milky PAGE gel-what gives?? Robert Preston
Primer stocks that fail to PCR over time Robert Preston
PCR: Why is my TAQ unfaithful Robert Preston
Flagging or hexaHis tag? Elmer M. Price
GST fusion prot.- HELP Kevin Pumiglia
Help! Western problems! Kevin Pumiglia
test R.I.Lewis at Swansea.ac.uk
Genbank Search Result?????? RAA
TECHNE thermal cyclers RCBaird
Chemiluminescence and the PhosphorImager? RNeubig at umich.edu
Question on chillies ROGERSH
PCR with radioactive nucleotides ROSS at SCI.WFEB.EDU
Info about albumin-bound palmitate Eric Raddatz
Antibodies to beta-lactamase? Uttam Rajbhandary
5'-end labeling of 8mer oligo? Uttam Rajbhandary
DNase Footprinting Off a gelshift Thiruvamoor P. Ramkumar
DNase FOOTPRINTING Off a Gel-Retarded Complex Thiruvamoor P. Ramkumar
Using Glycogen to prec DNA??? Colin Rasmussen
gene sequencing-HELP Colin Rasmussen
How much DNA is in a single rat neuron???????? Colin Rasmussen
Vector sequences? Colin Rasmussen
Altered Sites II Mutagenesis Colin Rasmussen
chilis Dennis J. Reeder
Chemiluminescence and the PhosphorImager? Tanya Reichert
Transgenic constructs Peter B. Reiner
Direct sequencing of lambda phage dna RichWalko
Vectors of the Past? Paul Riggs
SmaI/XmaI digests Paul Riggs
Help Needed for Oocyte RNA Expression Study RiverSloth
Immunogold staining ? Rob
Source for Hygromycin B Brian Robertson
Need help for emoving glycogen from DNA solution Antonio Rodriguez-Ariza
DNA purification problem: glycogen Antonio Rodriguez-Ariza
RECHERCHE LABO POUR UNE THESE : BIOLOGIE/BIOCHIMIE Monique Rogard
gene sequencing-HELP Francoise Roquet
cDNA library construction kits Mark Rose
Alkaline PFGE Elizabeth Rosenberg
help for oligo hybridization Janet E. Rubin
best way to remove PCR-primers Peter Rudolph
Odd problem with RAPD sequencing Robert Rumpf
DNA/Protein analysis software Ed Rybicki
Help chemiluminescent Immunoprecipitation SRINI at OPUS.MCO.EDU
PCR trouble: inverted repeat template NAMRATA SRIVASTAVA
O.C.T.? Steven L. Sabol
RNA isolation from PANCREAS!!! Steven L. Sabol
non-radioactive RNAse protection assays Jeffrey D. Saffer
advice on phosphorimager purchase Roland J Saldanha
Where can I get EcoA? Klaus Salger
Genomic DNA from E.coli without phenol or chloroform?? Klaus Salger
help sequence from puc Klaus Salger
Dumb question (?) re cycle sequencing of PCR products Klaus Salger
gene sequencing-HELP Klaus Salger
The Case of the Vanishing Intron Klaus Salger
pRSET sequence wanted Klaus Salger
Advice on GeneRunner Klaus Salger
S&S Rad Free system Klaus Salger
New DNA stain Klaus Salger
Vector sequences? Klaus Salger
HELP! RIBOPROBES. Klaus Salger
cloning problem Klaus Salger
BLOCKING SLN FOR DIG-SYSTEM!! Klaus Salger
problems in purification of small DNA fragments Klaus Salger
Primer Extension Claudia Salinas
Question on chillies Bill Salsgiver
Chemiluminescent assay for GUS Deborah A. Samac
U937 monocyte suspension culture adhering to flask. Why?! Mr. P.A. Sansom
re-probing nylon filters Leo Schalkwyk
registered trademark insignia Richard Schifreen
O.C.T.? Jennifer Schmitt
Thanks for OCT info Jennifer Schmitt
Oligos to filters Steve Schramm
antibody for enterokinase site Alan Schulman
DNAse contamination Hank Seifert
help w/ ecor1 contam! Sunil Shaw
New DNA stain Glen Shearer
PCR primer mismatches Dale R Shepard
cdk2 Yoshiyuki Shibata
PCRing 5' UTR using a cDNA plasmid? Mari L. Shinohara
Question on chillies Sandy Silverman
(none) Minati Singh
(none) Minati Singh
Ampicillin inactivation temp. Michael Skidmore
Soliciting Comments on Sequence Analysis Software Michael Skidmore
Good in situ hybridisation system? Rod Snowdon
UREA-GEL RECEPE WANTED Oliver Sorgenfrei
cDNA libraries primed with specific probes J. David Spafford
Antifoam A sterilisation?? Anne Spang
green fluorescent protein tag Anne Spang
Citrate Synthase Anne Spang
Help! Western problems! Anne Spang
human citrate synthase Anne Spang
Protean II glass breaking Anne Spang
DNAStar Program(s) Donald L. StGermain
Perplexing result with site-directed mutagensis Donald L. StGermain
T3/T7 PRIMER QUESTION!!! Darren Stauth
Photodocumentation Stefan Einar Stefansson
Test dont read Stephen R. Lasky, Ph.D.
gelatin filters Stephen R. Lasky, Ph.D.
In vitro translation kit? Stephen R. Lasky, Ph.D.
Is there any inexpensive way to separate RNA from DNA? Stephen R. Lasky, Ph.D.
How to separate plasmid DNA from Chromosome DNA? Stephen R. Lasky, Ph.D.
Rf: EDTA in cell fractionatio Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
chilis Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
How to Calculate Oligo MW? Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
What is 'gel mobility shift assay'? Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
test Please ignore Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
electrophoresis of nuclear extracts Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
chilis Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
chilis Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
Antibody warning Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
gene sequencing-HELP Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
U937 monocyte suspension culture adhering to flask. Why?! Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
Sequencing Gel Fixation Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
Chili Follow-up Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
chilis Ed Stephenson
Southern blot nightmare Ed Stephenson
Stability of Heparin B Stickney
Asksam? Satyam Subrahmanyam
Returned mail: User unknown Mail Delivery Subsystem
Synthetizing probes with Taq Zal Suldan
gst elution Zal Suldan
Molecular Biology Clip-Art Zal Suldan
Antifoam A sterilisation?? Dai Suter
Luciferase Problems? Clemens Suter-Crazzolara
Use of Amersham's Hybond N+ Clemens Suter-Crazzolara
Refolding urea-solubilized protein Clemens Suter-Crazzolara
ribo- or RPL probe ? Clemens Suter-Crazzolara
pET and ampicillin Clemens Suter-Crazzolara
Use of Amersham's Hybond N+ Clemens Suter-Crazzolara
Carbohydrate against DNA NICHOLAS THEODORAKIS
discrepancies between cDNA and protein sequences NICHOLAS THEODORAKIS
CHEMILUMINESCENT SYMPOSIUM TROPIX at BIOTECHNET.COM
Chemiluminescent Symposium TROPIX at BIOTECHNET.COM
SmaI/XmaI digests Song Tan
calculating extinction coefficient from protein sequence ??? Song Tan
pET vectors Song Tan
DNase FOOTPRINTING Off a Gel-Retarded Complex Song Tan
DNAse contamination in plasmid preps George Theodoris
Sequencing Gel Fixation George Theodoris
SmaI/XmaI digests George Theodoris
advice on phosphorimager purchase Ross Thomas
discrepancies between cDNA and protein sequences Michael R. Thompson
betagalactosidase antibodies ToddS41
HA epitope TAG: is 12CA5 the only Ab available? ToddS41
Vancouver Biotech Phone #/Address ToddS41
RNA content of single cells? Kai-M. Toellner
PCR: Why is my TAQ unfaithful Kai-M. Toellner
Homebrew saline for Contact Lenses Anthony Tomlinson
Soliciting Comments on Sequence Analysis Software Anthony Tomlinson
Electroporators: what brand? Anthony Tomlinson
Sequencing Gel Fixation Anthony Tomlinson
Luciferase detection Enrico Tongiorgi
5'-end labeling of 8mer oligo? Joanne Tornow
Help on pIBI30 Joanne Tornow
S&S Rad Free system Huu Minh Tran
Prep remains of mummies? Steve Tredwell
Bulk prep problems Beverly Trimpe
Silver staining of Native PAGE Sergey Troianovsky
gum arabic John True
gum arabic John True
DNA extraction after acid-phenol RNA prep James Tsuruta
Imaging Systems J. Tudor
"FSCP" Bruce J. Turner
Help, Sequence of pBMGNeo? Jeffrey Upperman
discrepancies between cDNA and protein sequences Chris Upton
epithelial cell culture John D. Valentich
...no subject... Jacqueline Vanderstappen
ABI 373 DNA sequencer Jacqueline Vanderstappen
Enhancer characterization Varyc
Markers Varyc
Clogged G-amidite lines Varyc
Molecular Genetics Ethicist Varyc
Need Advice on Film Recorders Paul A. Vescio
Psoralen Paul A. Vescio
Need human DNA libraries Jost Vielmetter
HELP: Pfu and Capillary PCR - Idaho Tech. Dick Vogt
Where to get Kirromycin Bjoern Voldborg
Where can I get KIRROMYCIN? Bjoern Voldborg
Where can I get KIRROMYCIN? Bjoern Voldborg
What is Resin in Magic mini-preps? WICKHAM,JOHN
Anyone know about pDK101? Matthew Wakefield
Globomycin FJ van der Wal
Antibodies to beta-lactamase? FJ van der Wal
Where Can I Buy A Hybridization Oven? Christine Ward
Need Primer Extension (of RNA) Info/Help Christine Ward
QUESTION: Design of DNA hybridization probes Todd Wareham
How to Calculate Oligo MW? Lawrence Washington
oligo purification on hplc Lawrence Washington
ALF DNA Sequencer Discussions Lawrence Washington
Neuronal cell line with glutamate receptors?? Charles E. Weaver
How much DNA is in a single rat neuron???????? Charles E. Weaver
New DNA stain Keith G. Weinstock
Mouse chromosomal location blots--NEEDED William Milford Rideout III Wellcome-CRC
good source for enterokinase? Malcolm White
verabrate expression vectors Sheryl White
Do you need supercoiled pDNA for ds-sequencing? Roger Wiegand
minipreps for sequencing Roger Wiegand
How to Calculate Oligo MW? Roger Wiegand
unable to ppt. protein using ammonium sulphate Roger Wiegand
ABI Support software for automated sequencing Roger Wiegand
Sequencing Gel Fixation Wiggy
What is 'gel mobility shift assay'? D. P. Wijesinghe
Nylon membrane blues Julie Wilberding
PCR: Why is my TAQ unfaithful Julie Wilberding
faulty southern hybridzation Julie Wilberding
?best purification of 186bp DNA fragment from agaraose Kimberly Ann Winkeler
help:how tomake 250ug 130bp fragment? Dana Wolle
Purifying a trancripxn factor using biotinylated DNA David Wong
Gel documentation Surasakdi Wongratanacheewin
a problem in protein purification Jim Woodgett
pJAY3 Vector Jim Woodgett
Looking for Balantidium culture Andre-Denis G Wright
Sorry wrong group! Andre-Denis G Wright
"LONG" RT/PCR Steve Wylie
Mussel tissue needed Jane Wyllie
Mussel tissue needed Jane Wyllie
ASAP Request - Heat Shock Info X-Man
Protean II glass breaking Xiao-Ru.Wang
Is there any inexpensive way to separate RNA from DNA? Xu, Liang
How to separate plasmid DNA from Chromosome DNA? Xu, Liang
Need advice on soybean protoplast Nanfel Xu
?best purification of 186bp DNA fragment from agaraose Ian A. York
Linear vs. Circular DNA Ian A. York
pH management Ronald Young
Separation of chromosome and plasmid Youngshik
best way to remove PCR primers Paquette Yves
What is spermidine??? Paquette Yves
best purification of 186bp DNA fragment from agarose Paquette Yves
best way to remove PCR primers MAURICE ZAUDERER
Thermojet Zainul Zain
RNAse ZAP Zeiler, Brian M'bio
Direct cloning of a bacterial gene Shula Zhang
Direct cloning of a bacterial gene (fwd) Shula Zhang
rho termination suppression=suppression by tRNA? a
Restriction enzyme HgiEII aairaksinen at ktl.fi
Stably transformed mammalian cells *HELP* acer
Vero, COS or human cell expressing T7 RNA polymerase??? acer
Monoclonals for influenza HA (mt sinai strain) *HELP* acer
ssDNA prep for mutagenesis only getting helper phage back acer
GST fusion prot.- HELP aduarte at vax.oxford.ac.uk
When do you starve cells after transfection? aeschba at fmi.ch
Luciferase sensitive to dilution aeschba at fmi.ch
Artifacts in silver staining ahung at ASRR.ARSUSDA.GOV
Plate viewer ahung at ASRR.ARSUSDA.GOV
info wanted on Dynal magnetic beads (efficiency of extraction) ajayaram at pearl.tufts.edu
Vent PCR conditions awalley at molbiol.ox.ac.uk
Vent PCR problem resolved - Thanks! awalley at molbiol.ox.ac.uk
TECHNE thermal cyclers dudy bar-zvi
Eppendorf Master Cycler 5330? dudy bar-zvi
RECOMMENDATION FOR FREEZE DRYERS dudy bar-zvi
Large-ish vectors that won't behave bcraft at darwin.bio.uci.edu
chilis bdl
How to Calculate Oligo MW? beavis
How to Calculate Oligo MW? books at mcvax4.d48.lilly.com
PCR with radiactive nucleotides brito at WUSTLB.WUSTL.EDU
Long Ranger brito at WUSTLB.WUSTL.EDU
Karyotype protocol for amphibian cell line Rachel bub
lambda packaging kits campcl at ubvms.cc.buffalo.edu
problems in purification of small DNA fragments carver cathy
(none) ccheon at CAMD2.KKPCR.RE.KR
PCR of formalin fixed tissues chelack at admin3
BLOCKING SLN FOR DIG-SYSTEM!! chengs1 at jaguar.uofs.edu
genomic library-probe & condition chien at mbcf.stjude.org
Cleaning Electroporation Cuvettes chouy at TCPLINK.NREL.GOV
PROMOTER BANK chouy at TCPLINK.NREL.GOV
Southern blot nightmare converrl at ucbeh.san.uc.edu
Alu sequences? converrl at ucbeh.san.uc.edu
expression constructs & ORF's converrl at ucbeh.san.uc.edu
cloning problem converrl at ucbeh.san.uc.edu
Ampicillin inactivation temp. Aurel d'etre
Phosphorimager brazelton anthony d
Phosphorimager brazelton anthony d
wizard dna clean up d.a.bailey
GST fusion protein won't elute bipin dalmia
gst elution bipin dalmia
inducible mammalian expression vector davis at wehi.edu.au
AUSTRALIA, Sidney, St. Vincent's Hospital, Dr. J.A. Eisman et al?? davis at wehi.edu.au
Antibody Production with synthetic Multiple Antigenic Peptides (MAPs) Anne deVaux
UV Source for SYBR GREEN? dhanzel at delphi.com
Level 2 Biosafety dkatz at doane.edu
Silver staining of Native PAGE dkim at unm.edu
HOME-MADE GENECLEAN KIT dkim at unm.edu
"frowning" within a lane dkim at unm.edu
Hyb-Aid nylon net dkim at unm.edu
non-radioactive RNAse protection assays dkim at unm.edu
inducible mammalian expression vector dirk dobbelaere
gross chromatin structure michelle or doug......
Looking for nuclear run-off assay help michelle or doug......
dynabead mRNA purification michelle or doug......
MDBK electroporation peter s. evans
my a amil adress faruk
Antibodies to beta-lactamase? fernanrc at ucbeh.san.uc.edu
PCR primer design with RE sites nicola fidler
G1/S hela cDNA library gao at mbcf.stjude.org
silver-staining DNA & cloning gc
milky PAGE gel-what gives?? gc
E.coli natural isolates christian a geltinger
U937 monocyte suspension culture adhering to flask. Why?! giwamot at indycms.iupui.edu
DNAse or nuclease assay mike grove
gelatin filters for EtBr photography gustilo at pobox.upenn.edu
gelatin filters for EtBr photography gustilo at pobox.upenn.edu
Clogged G-amidite lines gustilo at pobox.upenn.edu
quest for lipofectin protocol denis guttridge
pET and ampicillin jim hartley
milky PAGE gel-what gives?? heithem at odin.mda.uth.tmc.edu
random-primed cDNA library from chicks Domingos henrique
In vivo labelling of nucleic acids (non-radioactive) herzer at urz.unibas.ch
Breast cancer cell line needed herzer at urz.unibas.ch
Dictating DNA sequences to a computer hey at mbcl.rutgers.edu
<None> jenfedaque at samba.cnb.uam.es
Need help on operon/protein fusions jenfedaque at samba.cnb.uam.es
How much DNA is in a single rat neuron???????? jfh
Where can I obtain EcoA? kamin johnson
Where can I get EcoA? kamin johnson
ds DNA sequencing surefire protocol wanted. jr at dna.bio.warwick.ac.uk
Tbr polymerase jtornow at whale.st.usm.edu
recovery of oligos from solution jurgenson at cobra.uni.edu
Subcellular Fractionation k.c.breen at dundee.ac.uk
rat random cDNA wanted k.d.preuss
pRSET sequence wanted k.d.preuss
signal sequences? k.d.preuss
dead glycerol stocks? k.d.preuss
plasmid prep with G+ bacteria kannan at kuhub.cc.ukans.edu
Purification of cosmid DNA kannan at kuhub.cc.ukans.edu
Phosphorimager kevinsabith at delphi.com
Help! low recovery yeild of geneclean isaac kim
chilis kitchingman at mbcf.stjude.org
Sodium Acetate pH 4 lidaniel
Promega technical support lmgm at biomserv.univ-lyon1.fr
Help! Western problems! lmrc.
Help! Western problems! lmrc.
Problem with the Rad-Free system lmrc.
Psoralen lmrc.
a problem in protein purification yuling luo
a problem in protein purification yuling luo
Who sells myc monoclonal Ab? yuling luo
fitc labeling lw75
cholesterol binding lw75
Heparin in PCR? macklon at acs.ucalgary.ca
help sequence from puc macklon at acs.ucalgary.ca
gene sequencing-HELP macklon at acs.ucalgary.ca
'Good' Buffer with pKa of 5.3-5.4? mamber
BIOTECHNIQUES paolo de marco
PROMOTER BANK paolo de marco
Spectrophotometer Help john markwell
Izinta mazella at naxos.unice.fr
TSA 201 ??? mazella at naxos.unice.fr
Substrates for B-GUS and B-GAL ? mbridges at molbiol.ox.ac.uk
HOME-MADE GENECLEAN KIT mbxspd at unicorn.nott.ac.uk
pET and ampicillin mbxspd at unicorn.nott.ac.uk
C-terminal HIS tag/GST/myc fusion proteins mbxspd at unicorn.nott.ac.uk
Help! low recovery yeild of geneclean mbxspd at unicorn.nott.ac.uk
gelatin filters mcgraw at BSCR.UGA.EDU
chilis mdcabl at cc.newcastle.edu.au
Post-doctoral fellowship for a US national in England? merlin
Electroporators: what brand? mike at sgi1.fels.temple.edu
gene and enhancer mluo at cc.memphis.edu
Nightmare of 3'-RACE mmai at kean.ucs.mun.ca
Refolding urea-solubilized protein mmp at bioc.rice.edu
Origin of Amber codon name moulard at evalgb.geneti.uv.es
Origin of amber codon name moulard at evalgb.geneti.uv.es
AUTOMATED PCR SEQUENCING mwatson at uniwa.uwa.edu.au
DNA from fixed tissue neale at mbcf.stjude.org
In vitro translation kit? neale at mbcf.stjude.org
PCR: Why is my TAQ unfaithful neale at mbcf.stjude.org
Purine Nucleoside Phosphorylase source andy nixon
westerns using monoclonals nussbaum at neurochem.u-strasbg.fr
galactosyltransferase nussbaum at neurochem.u-strasbg.fr
Best/preferred methods ?? ott at wmvx.lvs.dupont.com
pET and ampicillin pathology at mscf.med.upenn.edu
PCR from dried sequencing gels pattonaj%phvax.dnet at sb.com
in situ PCR pattonaj%phvax.dnet at sb.com
WGA agarose pattonaj%phvax.dnet at sb.com
milky PAGE gels pattonaj%phvax.dnet at sb.com
quest for lipofectin protocol carlos h. pedemonte
Help with biotin-labeled DNA probes carlos h. pedemonte
luciferase detection carlos h. pedemonte
CDTA john peloquin
phenanthrene degredation bradley plantz
(none) pnh at ncifcrf.gov
(none) pnh at ncifcrf.gov
PCR with radioactive nucleotides pnh at ncifcrf.gov
Frequently Asked Question (FAQ) list pnh at ncifcrf.gov
SYBR Green I Filter pnh at ncifcrf.gov
DNAse contamination in plasmid preps pnh at ncifcrf.gov
gelatin filters pnh at ncifcrf.gov
best purification of 186bp DNA fragment from agarose pnh at ncifcrf.gov
Who sells myc monoclonal Ab? popa0077 at PO-Box.McGill.CA
no subject (file transmission) pzhang
no subject (file transmission) pzhang
Oligo pzhang
5'-end labeling of 8mer oligo? pzhang
(none) r3603212
Just checking... ranga at vigyan.iisc.ernet.in
Help! Ligation of phophatased DNA... rcaspi at ucsd.edu
advice on phosphorimager purchase rdkortschak at science.adelaide.edu.au
UREA-GEL RECEPE WANTED reichhart at astorg.u-strasbg.fr
DNA/Protein analysis software kenneth garson cancer research
PCR/single 3' mismatch kenneth garson cancer research
LA-PCR ends? rhubner at molbiol.ox.ac.uk
Breast cancer cell line needed rlr
Breast cancer cell line needed rlr
Direct sequencing of lambda phage dna rmewing at vax.oxford.ac.uk
DNA strand separation rnwil003 at uctvax.uct.ac.za
Help! Dye terminator sequencing rnwil003 at uctvms.uct.ac.za
SmaI/XmaI digests rpgrant at molbiol.ox.ac.uk
GST Problems - an idea rpgrant at molbiol.ox.ac.uk
Summary: PCR of GC-rich DNA sbaker at molbiol.ox.ac.uk
Advice on GeneRunner schoenfeld at MSSCC.MED.UTAH.EDU
Substrates for B-GUS and B-GAL ? schupp at nauvax.ucc.nau.edu
Dumb question (?) re cycle sequencing of PCR products schupp at nauvax.ucc.nau.edu
Eppendorf Master Cycler 5330? schupp at nauvax.ucc.nau.edu
Lambda ZAP genomic library problems schupp at nauvax.ucc.nau.edu
PCR from dried sequencing gel? schupp at nauvax.ucc.nau.edu
Luciferase detection schupp at nauvax.ucc.nau.edu
SSCP analysis schupp at nauvax.ucc.nau.edu
Easy/CHEAP Genomic DNA silvia
verabrate expression vectors skoufos at nefeli.imbb.forth.gr
Nuclear Factor-DNA Binding Motifs skoufos at nefeli.imbb.forth.gr
Post-doctoral fellowship for a US national in England? skoufos at nefeli.imbb.forth.gr
HELP! Problems with the expression of HSP 70. sl16s at cc.usu.edu
HELP! RIBOPROBES. sl16s at cc.usu.edu
PCR-Primer-dimers sl6dp at cc.usu.edu
PRIMER-DIMERS in PCR sl6dp at cc.usu.edu
PCR-PROPDUCT PURIFICATION sl6dp at cc.usu.edu
Photodocumentation stefan at sickkids.on.ca
bionet.molbio.methds-reagnts swift at mcvax4.d48.lilly.com
Glycine buffer swjnev01 at uctvax.uct.ac.za
Odd problem with RAPD yasunari takami
DNA from formalin preserved tissue uks13mh
Radiolabelling proteins overexpressed in E.coli user unknown
Texas Red labeling of antibodies uvsbgsm at Msu.oscs.montana.edu
cell surface biotinylation uvsbgsm at Msu.oscs.montana.edu
glass microtiter plates -- commercially available? vinny
Southern blot nightmare vitor warwar
Help Needed for Oocyte RNA Expression Study wch190 at lulu.acns.nwu.edu
pET and ampicillin wch190 at lulu.acns.nwu.edu
pET vectors wch190 at lulu.acns.nwu.edu
pET vectors wch190 at lulu.acns.nwu.edu
cAMP binding protein wch190 at lulu.acns.nwu.edu
Hyb. probe c-jun/c-fos for dog? weindel at mzdmza.zdv.uni-mainz.de
cDNA for a housekeeping gene? wiens at cobra.uni.edu
easy band isolations sa wiese
Prof. Jeffrey D. Palmer wochniak at sggw.sggw.waw.pl
Gus x at x.x.ch
(none) xia at WARREN.MED.HARVARD.EDU
Wanted: testicular/adrenal gland library michele young
SSCP-PCR & methylmercury hydroxide zoodr at zoo.latrobe.edu.au

Last message date: Wed Nov 30 23:42:50 EST 1994
Archived on: Sun Mar 6 20:19:29 EST 2005

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