small DNA fragment recovery
salger at wap18.zi.biologie.uni-muenchen.de
Sun Oct 2 13:12:50 EST 1994
: > Low-melt agarose method from Jim Graham (jgraham at bronze.ucs.indiana.edu):
: Dear Klaus,
: This sounds like a great method for isolating DNA fragments. Have you used
: it on larger fragments, eg 5kb?
: Also, do you place you tubes containing the melted agarose /DNA, in an
: isopropanol bath, in a -70 freezer?
: (m-brazil at uk.ac.mrc.nimr)
as far as I remember, the biggest fragment I tried was about 5kb
- no problem.
I usually freeze in liquid nitrogen, dry ice + ethanol does also work.
I didn't compare the yields but had enough fragment with either method.
Hope this helps
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e-mail: salger at zi.biologie.uni-muenchen.de
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