small DNA fragment recovery

Klaus Salger salger at wap18.zi.biologie.uni-muenchen.de
Sun Oct 2 13:12:50 EST 1994


 
: > Low-melt agarose method from Jim Graham (jgraham at bronze.ucs.indiana.edu):

: Dear Klaus,

: This sounds like a great method for isolating DNA fragments. Have you used
: it on larger fragments, eg 5kb? 

: Also, do you place you tubes containing the melted agarose /DNA, in an
: isopropanol bath, in a -70 freezer?

: Thanks
: Melanie
: (m-brazil at uk.ac.mrc.nimr)

Melanie,
as far as I remember, the biggest fragment I tried was about 5kb
- no problem.
I usually freeze in liquid nitrogen, dry ice + ethanol does also work.
I didn't compare the yields but had enough fragment with either method.
Hope this helps
  Klaus


Klaus Salger
Zoologisches Institut
AG MacWilliams
Luisenstr. 14
80333 Muenchen
Germany

phone : ++49 (0)89 5902 -502
FAX   :                 -450
e-mail: salger at zi.biologie.uni-muenchen.de




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