non-isotopic gel shift assays
salger at wap18.zi.biologie.uni-muenchen.de
Mon Oct 3 15:16:40 EST 1994
slee at opal.tufts.edu wrote:
: Hi. Does anyone have a protocol for using a non-isotopic method of
: doing gel shift assays? Please direct me to either an article or
: the name of a commercial kit. If you have direct experience with
: the non-isotopic labeled oligo's, what is their stability--days?
: month? at 4 C, -20 C or -80 C?. Thanks for the info.
Boehringer Mannheim sells the "DIG Gel Shift Kit".
BM says DIG-labeled probes are stable for at least 1 year at
-20 C. I don't have experience with oligos but I didn't
notice a decay with labled DNAs or RNAs within more then a year.
(No affiliation with BM...)
phone : ++49 (0)89 5902 -502
FAX : -450
e-mail: salger at zi.biologie.uni-muenchen.de
More information about the Methods