Hair Follicle DNA Fingerprinting
brwnng at CCWF.CC.UTEXAS.EDU
Mon Oct 3 15:23:15 EST 1994
Ann Kleinschmidt (akleinsc at bio7.alleg.edu) wrote:
: students who are not familiar with the use of radioactive compounds. Does
: anyone have a protocol for isolation of DNA from hair follicles? Could
: you also point me in the correct direction for kits for PCR fingerprinting
: that are not dependent upon radioactivity.
: Please send responses directly to me. My email address is
: akleinsc at alleg.edu
:Please post on the net also. I'd like to incorporate the info in a course.
We do a PCR experiment based on the DS180 typing kit from P/E Express for an
undergraduate biochemisty laboratory.
We use hair roots as the source of DNA. I do not know how many hair roots
you would need to get enough to do a restriction digest for fingerprinting.
If you would like a copy of our experiment, let me know.
Karen S. Browning, Ph.D. (email) brwnng at ccwf.cc.utexas.edu
Dept. of Chemistry and Biochemistry (fax) 512-471-8696
The University of Texas at Austin (phone) 512-471-4562
Austin, TX 78712
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