Problem with Native PAGE

[Pin Xu]

Hi, Netter,

I recently tried to purify an enzyme on native PAGE. I used Bio-Rad mini-gel
apparatus and Tris-Glycine (Laemmli) buffer system (without SDS). The problem
is I can never get a clean gel: vertical streak, band diffusion, protein
precipitate in the interface bewteen stacking gel and separating gel, etc, etc
etc. I wonder if there is a good protocol for Native PAGE?

Thanks,

P.Xu