secondary screening after differential display

[Shahram Mori]

pattonaj%phvax.dnet at sb.com wrote:
: I've used slot blots unstead of northern as I had lots of "positives" to
: screen and I thought I could get away with less RNA on a slot blot. I've 
: seen other people screen by reverse northern.
: While we're on the subject of DD. Is anyone getting it to work reliably?
: Every "positive" from the sequencing gel that I've rePCRed to date has been
: a false positive in that it lights up in all samples on secondary screening.
: I realise that I need to get reproducible results on the sequencing gel but
: I'm finding that hard, however, careful I try to be. Any suggestions would
: be gratefully received. Many thanks

: Amanda Patton

This is just an idea but maybe using one random 10mer will give you a more
reproducible result.
cheers,
--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003