downward DNA transfer?

Cornelius Krasel krasel at alf.biochem.mpg.de
Tue Oct 4 15:17:35 EST 1994


LEES at SASK.USASK.CA wrote:
: Unlike upward standard DNA transfer technique, this downward has a big 
: chance to be short-circuit between upper and lower constructs. How do 
: you make construct above the gel? If it is not too thick enough, wick 
: ppaper may have chance to contact lower paper towel construct which i
: just below the gel.

I surrounded the gel with stripes of Parafilm and put the upper towels on
that. No problems with short circuits.

--Cornelius.

--
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de                 fax: +49 89 8578 3795 */
/* "People are DNA's way of making more DNA." (Edward O. Wilson, 1975)    */



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