downward DNA transfer?

[Cornelius Krasel]

LEES at SASK.USASK.CA wrote:
: Unlike upward standard DNA transfer technique, this downward has a big 
: chance to be short-circuit between upper and lower constructs. How do 
: you make construct above the gel? If it is not too thick enough, wick 
: ppaper may have chance to contact lower paper towel construct which i
: just below the gel.

I surrounded the gel with stripes of Parafilm and put the upper towels on
that. No problems with short circuits.

--Cornelius.

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/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
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