RT-PCR question

Gerri Newfry gnew at orion.it.luc.edu
Tue Oct 4 11:41:06 EST 1994


I use random primers for the RT step, which are supposed to (according to 
clontech) 
work better.  Also, I started using a cDNA cycle kit from Invitrogen.

Good luck,

Gerri

 Adi Santoso 
(santoso at badlands.NoDak.edu) wrote:
: i try several time RT-PCR with 3' primer using oligo dT (16 mers) just 
: never work. I check all the reagents and template RNA, they are all 
: oke...!!! any suggestion for me.  I just almost give up with it..

: ADI
: Dept. of Biochemistry
: North Dakota State University

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		gerri...gnew at orion.it.luc.edu				   




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