Aggregation of membrane proteins
maraman at ksu.ksu.edu
Tue Oct 4 11:51:23 EST 1994
Hi, I am trying to purify a membrane protein using antibody affinity chromatography. After elution I test them on SDS-PAGE and silver stain them to check for purity. But the proteins seems to aggregate & never enters the stacking gel. If at all it enters into the separating gel it runs a little high than where it is supposed to be. I do have detergent (nonionic) in the elution buffer. Does anybody had a similar problem & know how to troubleshoot?.
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