ANTIBIOTIC STORAGE

Martin Kennedy mkennedy at chmeds.ac.nz
Tue Oct 4 23:36:19 EST 1994


In article <36gvo4$6dj at mserv1.dl.ac.uk>, POPOVA <ANDREI.POPOV at afrc.ac.uk> writes:
> CHLORAMPHENICOL - 34 MG/ML    ETHANOL    -20 C VERY STABLE
> KANAMYCIN         10 MG/ML    WATER      -20 C LIMITED TIME
> TETRACYCLINE       5 MG/ML    ETHANOL    -20 C  VERY STABLE
> AMPICILLIN        10 MG/ML    ETHANOL    -20 C VERY STABLE
> 
> I USE LB OR 2xYT 2% AGAR PLATES STORED AT +4C AND SUPPLEMENT WITH
> ANTIBIOTIC (S) JUST BEFORE USE
> 
> ANDREI POPOV

I do the same as Andrei, mainly because ampicillin is not stable at 4 C,
and plates containing amp that have been stored in the cold room for 
more than a week will grow amp sensitive bacteria perfectly happily.

Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ



More information about the Methods mailing list