Best method for blunting ds DNA?

Klaus Salger salger at wap18.zi.biologie.uni-muenchen.de
Tue Oct 4 17:19:57 EST 1994


Bernard Murray (bernard at elsie.nci.nih.gov) wrote:
<snip>
: Basically, what is your favourite/easiest/cheapest/most effective way of
: blunting a DNA fragment? - with 5' and/or 3' overhangs
<snip>

Bernard, I use Klenow for fill-ins (tradition) and T4 DNA polymerase
for everything else. So if I have 3' overhangs or 3' and 5' overhangs
I ad just T4 polymerase as it has both 5'-3' polymerase activity and
3'-5' exonuclease activity (much more then Klenow). It worked whenever
I tried it.
Hope this helps
  Klaus

--

Klaus Salger
Zoologisches Institut
AG MacWilliams
Luisenstr. 14
80333 Muenchen
Germany

phone : ++49 (0)89 5902 -502
FAX   :                 -450
e-mail: salger at zi.biologie.uni-muenchen.de



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