Oligo dT for RT?

POPOVA ANDREI.POPOV at afrc.ac.uk
Wed Oct 5 12:52:40 EST 1994


Is there an optimal length for oligo dT to prime a reverse transcriptase
reaction?  We want to synthesize such a primer.  Any benefit in a range of
lengths? Any reference?  Thanks

--
***********************************************************************
Elwyn Loh
University of Pennsylvania
loh at mail.med.upenn.edu 

DEAR ELWYN,
I USE OLIGO dT 12-18 MER FROM PHARMACIA AT 40 C WITH
SUPERSCRIPT RT (BRL).  MAYBE 18-20 MER WOULD BE EVEN
BETTER FOR THIS TEMP. RT-PCR PRODUCTS CORRESPOND TO
cDNA ABOUT 200O BP.

BEST WISHES
ANDREI POPOV




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