Rf: In search of the "Holy manual"
logand at msdos.montpellier.inra.fr
Wed Oct 5 07:45:51 EST 1994
In Article <36to14$4dg at mserv1.dl.ac.uk>, SPAGNOL at PLAUTO.CSATA.IT wrote:
>I desperately seek a laboratory manual in the field of proteins which (ideally)
>should be what Maniatis is for nucleic acids.In short it should consist of well
>explained general principles about protein biochemistry and immunochemistry and
>accurate protocols joined in the same book.Does someone outthere know a book
>Sincerely ,Giorgio: a molecular biologist who is paying the price of
> I know of two books that are excellent for theory and practice of
>protein purification, if this is what you mean.
>(1) Robert Scopes _Protein Purification_. I have the second edition and
>don't know if there is a third edition. It is publised by Springer-Verlag
>(2) Murray Deutscher (ed) _Guide to protein purification_. This is from
>the Methods in Enzymology series and is published by Academic Press.
> If anyone knows of any other texts, I would also be interested in
>knowing about them.
>Plant research group
Another very good book on the immunochemistry side of things (also contains many
basic protein techniques allied to immunology) is Antibodies: A laboratory
manual by Ed Harlow and David Lane published by Cold Spring Harbour Press. Very
similar to Sambrook et al. in approach.
David C. Logan
More information about the Methods