Phosphoimagers vs Instantimager

macIIci raffa at biocell.irmkant.cnr.rm.it
Wed Oct 5 09:08:52 EST 1994


In article <phef-0410941636030001 at bglab_1.botany.duke.edu>
phef at acpub.duke.edu (Peter Heifetz) writes:


> 
> Not quite true.  The screens themselves are very linear out to 5 logs
> (much better than film, for example).  The response of the screen
> _with_time_ to a given signal, is however, not linear.  Quantification can
> be done quite accurately with a Phosphorimager with a little attention to
> the limitations of the machine, correct use of standards and internal
> controls, and a good working knowledge of the proper techniques of image
> analysis.  Unfortunately, these topics are not usually covered by the
> machine/software manuals or the technical sales reps who set up the
> imagers in your lab...
> 
> -- 
> Peter Heifetz
> Duke University
> Developmental, Cell, and Molecular Biology Group


Is there a good reference for time-dependent response of
PhosphorImager?

Raffaele Matteoni
Inst Cell Biology
CNR
I-OO137 Roma Italy



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