Phosphoimagers vs Instantimager

macIIci raffa at
Wed Oct 5 09:08:52 EST 1994

In article <phef-0410941636030001 at>
phef at (Peter Heifetz) writes:

> Not quite true.  The screens themselves are very linear out to 5 logs
> (much better than film, for example).  The response of the screen
> _with_time_ to a given signal, is however, not linear.  Quantification can
> be done quite accurately with a Phosphorimager with a little attention to
> the limitations of the machine, correct use of standards and internal
> controls, and a good working knowledge of the proper techniques of image
> analysis.  Unfortunately, these topics are not usually covered by the
> machine/software manuals or the technical sales reps who set up the
> imagers in your lab...
> -- 
> Peter Heifetz
> Duke University
> Developmental, Cell, and Molecular Biology Group

Is there a good reference for time-dependent response of

Raffaele Matteoni
Inst Cell Biology
I-OO137 Roma Italy

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