UV-crosslinking of DNA-protien complex
lyc at IBMS.SINICA.EDU.TW
Thu Oct 6 01:59:08 EST 1994
We have obtained a BromodU-labelled oligonucleotide and planned to do
UV-crosslinking experiments. As we know that BromodU is light-sensitive.
Do we need to perform all the experiments in the dark including
oligonucleotides annealing, DNA purification, radioactive labelling and
protein binding reaction before UV-crosslinking?
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