UV-crosslinking of DNA-protien complex

[L.Y.Chau]

 Dear netters:
 We have obtained a BromodU-labelled oligonucleotide and planned to do 
 UV-crosslinking experiments.  As we know that BromodU is light-sensitive.  
 Do we need to perform all the experiments in the dark including 
 oligonucleotides annealing, DNA purification, radioactive labelling and 
 protein binding reaction before UV-crosslinking?

 Jonghwei Pang