UV-crosslinking of DNA-protien complex

L.Y.Chau lyc at IBMS.SINICA.EDU.TW
Thu Oct 6 01:59:08 EST 1994


 Dear netters:
 We have obtained a BromodU-labelled oligonucleotide and planned to do 
 UV-crosslinking experiments.  As we know that BromodU is light-sensitive.  
 Do we need to perform all the experiments in the dark including 
 oligonucleotides annealing, DNA purification, radioactive labelling and 
 protein binding reaction before UV-crosslinking?

 Jonghwei Pang




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