Rf: Antibodies to KDEL or kXXkx peptides
logand at msdos.montpellier.inra.fr
Thu Oct 6 05:05:03 EST 1994
>Dear netters: I looking for good antibodies that recognizes either MOST soluble
>ER proteins carrying K/H/RDEL tetrapeptide C-termini mottif, or antibody that
>recognizes the ER membrenal proteins carrying the KxxKx mottif. I am working
>in plant system and
> wish to look what happens to such proteins when i am using antisense plants
>for ERD2 (the putative receptor for ER soluble proteins).
> Thanks in advance, Maor
>Plant Res. Lab, MSU East lansing MI
>E-mail 22693.mgr at msu.edu .
Contact Richard Napier at HRI, East Malling Kent UK. He has a very good
monoclonal which is anti-KDEL. His full address is:
Dr R.M. Napier
Tel. (as calling from in UK) 0732 (area code) 843833 Fax 0732-849067
Email is RNAPIER at AFRC.ac.uk (but fax would get a quicker response since I think
he does not necessarily check his Email daily).
I have used it myself when I was a postdoc there. Epitope mapping carried out
too on this antibody.
As for the ER signal KXXKX I don't know but would be very interested in the
responses you get.
David C. Logan
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