35S labeling E coli
pablo at bioc.rice.edu
Thu Oct 6 16:28:00 EST 1994
I need to 35S label (met and cys) my favorite protein that I have in a
pET21 vector (IPTG-inducible), but I'm not sure how to do it. So, I've got
1. Should I add the label before or after I induce expression of my
protein with IPTG?
2. Do I have to use a synthetic medium without met and cys, or can I use
a rich medium like LB, 2xYT or TB?
Any and all help would be appreciated.
Thanks a million.
Paul Campbell (a.ka. pablo at bioc.rice.edu)
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