afc at gnv.ifas.ufl.edu
Thu Oct 6 10:26:11 EST 1994
In article <1994Oct6.072923.5562 at tower>, jwg at gnv.ifas.ufl.edu (MAD, MAD, MAD SCIENTIST) writes:
> In article <1994Oct5.163619.609 at chmeds.ac.nz>, mkennedy at chmeds.ac.nz (Martin Kennedy) writes:
>> In article <36gvo4$6dj at mserv1.dl.ac.uk>, POPOVA <ANDREI.POPOV at afrc.ac.uk> writes:
> question--How do you add the antibiotic to media that is
> already solidified? I would think one would have to add
> the antibiotic to the media just prior to pouring the plates
> and after the media had cooled to almost solidifying temperature.
<lots of stuff deleted>
Just pipette the antibiotic onto the plate and spread it around with
a cell spreader. It diffuses fast enough that it will reach the right
concentration before the cells start growing significantly.
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