ANTIBIOTIC STORAGE

[MAD, MAD, MAD SCIENTIST]

In article <1994Oct5.163619.609 at chmeds.ac.nz>, mkennedy at chmeds.ac.nz (Martin Kennedy) writes:
> In article <36gvo4$6dj at mserv1.dl.ac.uk>, POPOVA <ANDREI.POPOV at afrc.ac.uk> writes:
question--How do you add the antibiotic to media that is
already solidified?  I would think one would have to add 
the antibiotic to the media just prior to pouring the plates
and after the media had cooled to almost solidifying temperature.




>> CHLORAMPHENICOL - 34 MG/ML    ETHANOL    -20 C VERY STABLE
>> KANAMYCIN         10 MG/ML    WATER      -20 C LIMITED TIME
>> TETRACYCLINE       5 MG/ML    ETHANOL    -20 C  VERY STABLE
>> AMPICILLIN        10 MG/ML    ETHANOL    -20 C VERY STABLE
>> 
>> I USE LB OR 2xYT 2% AGAR PLATES STORED AT +4C AND SUPPLEMENT WITH
>> ANTIBIOTIC (S) JUST BEFORE USE
>> 
>> ANDREI POPOV
> 
> I do the same as Andrei, mainly because ampicillin is not stable at 4 C,
> and plates containing amp that have been stored in the cold room for 
> more than a week will grow amp sensitive bacteria perfectly happily.
> 
> Cheers,
> 
> Martin
> 
> NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
> NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
> NN  NN NN           Christchurch School of Medicine            ZZZ
> NN   NNNN              Christchurch, New Zealand              ZZZZZZZ