In article <hraaxl.4.00091AB1 at marc.cri.nz>, hraaxl at marc.cri.nz says:
>>Dear everyone
>>Heres the problem. I want to set up an assay system. The system has to have a
>flourescent product or produce light (eg like GUS or luciferase) and most
>importantly have a short as possible half life in the cell. So, GUS I believe
>might not be ideal because its supposed to have a long half life. Perhaps
>luciferase would be better?
>>So one question is, what is the half life of GUS and luciferase in the
>cell?
>>And secondly (and more importantly) if anyone knows of another enzyme (I have
>to be to get the clone as well) I could use to do the equivalent job I'd be
>very grateful.
>>Thanks
>>Tony Lough
>HortResearch
>New Zealand
>>CAT has a half life of about 50 hrs in mammalian cells and Luciferase
has a half life of about 3 hrs. I don't know about GUS. another potential
reporter gene is B-gal which I think also has a long half life but I
can't recall what it is.
Jeff Silverman
NCI, NIH
