half life of GUS, luciferase and other reporters?

Jeffrey Alan Silverman jeffs at elsie.nci.nih.gov
Fri Oct 7 09:04:28 EST 1994


In article <hraaxl.4.00091AB1 at marc.cri.nz>, hraaxl at marc.cri.nz says:
>
>Dear everyone
>
>Heres the problem. I want to set up an assay system. The system has to have a 
>flourescent product or produce light (eg like GUS or luciferase) and most 
>importantly have a short as possible half life in the cell. So, GUS I believe 
>might not be ideal because its supposed to have a long half life. Perhaps 
>luciferase would be better?
>
>So one question is, what is the half life of GUS and luciferase in the 
>cell?
>
>And secondly (and more importantly) if anyone knows of another enzyme (I have 
>to be to get the clone as well) I could use to do the equivalent job I'd be 
>very grateful.
>
>Thanks
>
>Tony Lough
>HortResearch
>New Zealand
>
>
CAT has a half life of about 50 hrs in mammalian cells and Luciferase
has a half life of about 3 hrs. I don't know about GUS. another potential
reporter gene is B-gal which I think also has a long half life but I
can't recall what it is.

Jeff Silverman
NCI, NIH



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