RT-PCR question

Simon Dawson mbxspd at unicorn.nott.ac.uk
Fri Oct 7 05:15:45 EST 1994


Hi there,
          We have tried RT-PCR using both oligo-dT and random hexamers
for the RT step and we have had a lot of trouble using oligo-dT (for
some reason!). We routinely use random hexamers nowadays for the RT
step followed by specific primers for the PCR step. Works every time,
always assuming we get our PCR profile set up right that is :)
Hope that is some use.

Simon.

Simon Dawson              Internet email: mbxspd at unicorn.nott.ac.uk
Dept. Biochemistry
Nottingham University
U.K.



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