RT-PCR question

[rhubner at molbiol.ox.ac.uk]

In article <Cx24Ep.4r5 at ns1.nodak.edu>, santoso at badlands.NoDak.edu (Adi Santoso) writes:

> i try several time RT-PCR with 3' primer using oligo dT (16 mers) just 
> never work. I check all the reagents and template RNA, they are all 
> oke...!!! any suggestion for me.  I just almost give up with it..
> 
> ADI
> Dept. of Biochemistry
> North Dakota State University

hi there,
 hmmm, with a 16mer your Tm can be about 32oC ONLY, right?
Greetings,
 Roland

ps. you can buy oligo(dT)25-30... and climb to 60oC...