SDS Problem

Simon Dawson mbxspd at unicorn.nott.ac.uk
Fri Oct 7 05:24:01 EST 1994


Hello,
      We don't seem to have this problem at all and we use the same
wash procedure quite a lot of the time. However, we dont hybridise
using Denhardts, salmon sperm DNA, polyA etc......we just use 6XSSC, 1%
SDS, 500µg/ml Heparin sulphate (standard grade i.e ordinary). The
heparin sulphate replaces all that messy Denhardts etc and works
beautifully.

Simon.

Simon Dawson              Internet email: mbxspd at unicorn.nott.ac.uk
Dept. Biochemistry
Nottingham University
U.K.



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