RT-PCR question

Shahram Mori smori at opus.nmsu.edu
Thu Oct 6 22:09:56 EST 1994


Shahram Mori (smori at opus.nmsu.edu) wrote:


: I agree completely with Annette. Use 6mers for cDNA synthesis ( 1hr at
: 25C) and forget oligo dt's. Make your library using random 10mers. 
: cheers,

I meant 35C not 25. Sorry!
cheers,


--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003





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