Coupling IgG via sugar residues
TOMAS.BRATT at MEDKEM.LU.SE
Sun Oct 9 07:00:23 EST 1994
Subject: Coupling IgG via sugar residues
From: Dave Pearton, pearton at stein1.u.washington.edu
Date: 8 Oct 1994 04:51:37 GMT
In article <3758gp$1ur at news.u.washington.edu> Dave Pearton,
pearton at stein1.u.washington.edu writes:
>I saw somewhere a method of coupling IgG molecules to a resin via their
>residues. This apparently relieves some of the problems about reduced
>through coupling at an incorrect orientation.
>Does anyone know which company was selling this product? Has anyone
>coupling method and might be prepared to comment on its usefulness? We
>trying to purify protein G.
>pearton at u.washington.edu
>pearton at unpsun1.cc.unp.ac.za
We have used Affi-gel Hz hydrazide gel from Bio-Rad with good experience.
The antigen binding capacity of the immobilized antibody is larger than
for example for CNBr-activated gel. However, for protein G purification I
beleive you could use most immobilization methods.
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