Coupling IgG via sugar residues

Tomas Bratt TOMAS.BRATT at MEDKEM.LU.SE
Sun Oct 9 07:00:23 EST 1994


Subject: Coupling IgG via sugar residues
From: Dave Pearton, pearton at stein1.u.washington.edu
Date: 8 Oct 1994 04:51:37 GMT
In article <3758gp$1ur at news.u.washington.edu> Dave Pearton,
pearton at stein1.u.washington.edu writes:
>I saw somewhere a method of coupling IgG molecules to a resin via their
sugar
>residues.  This apparently relieves some of the problems about reduced
binding
>through coupling at an incorrect orientation.
>
>Does anyone know which company was selling this product?  Has anyone
used this
>coupling method and might be prepared to comment on its usefulness?  We
are 
>trying to purify protein G.
>
>Yours,
>Dave
>pearton at u.washington.edu
>pearton at unpsun1.cc.unp.ac.za
We have used Affi-gel Hz hydrazide gel from Bio-Rad with good experience.
The antigen binding capacity of the immobilized antibody is larger than
for example for CNBr-activated gel. However, for protein G purification I
beleive you could use most immobilization methods.

best regards



More information about the Methods mailing list