clean GST antibodies source?????
T. S. Pillay
tpillay at ucsd.edu
Sun Oct 9 11:06:36 EST 1994
Subject: clean GST antibodies source?????
From: PRAMATHESH PATEL, patel_p at watson.bms.com
Date: 7 Oct 1994 09:37 EST
In article <7OCT199409375713 at watson.bms.com> PRAMATHESH PATEL,
patel_p at watson.bms.com writes:
> Does anybody know of a source for GST antibodies besides Promega. We
>tried the antisera purchased from promega. Unfortunately it is
>with an antibody that cross- reacts with a 70k heat shock protein of E.
>and you guessed it, our fusion protein MW is 70 kD. Surprisingly,
>tech. service confirmed this contamination as a matter of fact and felt
>it was not a big deal.
> Any help will be appreciated.
>Thanks in advance
>e-mail: Patel_P at BMS.com
Try Santa Cruz or Pharmacia. Pharmacia sells it as part of a kit
The best way is to raise your own from pure GST or purify some from
antiserum raised against a GST fusion protein using GST coupled to
More information about the Methods