PHAGE DNA KITS?
gshearer at whale.st.usm.edu
Mon Oct 10 09:02:25 EST 1994
Randall J. Cohrs (COHRS-R at Oberon.HSC.Colorado.edu) wrote:
: > Subject: PHAGE DNA KITS?
: > Are any kits or techniques available to purify very clean DNA from
: > phage preps prepared by the plate lysate method?
: I've prepared phage DNA from plate lysates by PEG-precipitation
: followed by twice banding on CsCl gradients.
: Also by PEG precipitation without banding
: but with SDS/proteinase K followed by
: phenol/chloroform/isoamyl alcohol and
: - - - for my money ($115 for 20 extractions) I now use Nucleobond from
: the Nest group (no affiliation)
: RJC in Denver
Rather than a kit......we've have very good results with a SDS/PEG
method c.f., Lockett. 1990. Analytical Biochem. 185:230. I can
send a protocol if you'd like.
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