Kunkel mutagenesis- Help needed
Yasha at bioch.tamu.edu
Mon Oct 10 09:19:10 EST 1994
In article <bmw-101094090231 at mi4.molbio.ku.dk>, bmw at biobase.aau.dk (Berthe
M. Willumsen) wrote:
> Did you check that your plaque development was dependent on your oligo?
> Often, contaminating RNA results in plaque formation, and these, of course,
> do not carry the mutation.
I've heard that contaminating DNA can result in plaque formation, but RNA?
We always run a negative control to help insure that the plaques we see
aren't due to contamination. I'd be very interested if you have more
information regarding problems with RNA in site-directed mutagenesis.
Texas A&M University
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