retroviral DNA preps

[Olav Hungnes]

Lorena C. Lim (altur at post.its.mcw.edu) wrote:
: Dear Netters:

: i need to know if naked retroviral DNAs are infectious. My P.I. said the DNA
: preps should all be done in the hood, which sounds silly to me. 

: i appreciate any reply

Yes, the retroviral DNA is in principle infectious. Cells transfected in 
culture will produce virus, an in light of recent data on in vivo 
expression of injected plasmid DNA (DNA vaccines) this could perhaps also 
happen with retroviral DNA inoculated into body cells. I vaguely remember 
someone saying that the DNA is about one million-fold less infectious 
than the virus itself. So don't splash it about.

Olav
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_______________________________________________________
Olav Hungnes                 olav.hungnes at embnet.uio.no
National Institute               Phone  (+47)22042200
of Public Health                 FAX    (+47)22353605
Oslo, NORWAY
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--
_______________________________________________________
Olav Hungnes                 olav.hungnes at embnet.uio.no
National Institute               Phone  (+47)22042200
of Public Health                 FAX    (+47)22353605
Oslo, NORWAY
_______________________________________________________