Why MOPS for RNA gels?
David S. Huen
smh1008 at cus.cam.ac.uk
Mon Oct 10 03:06:58 EST 1994
In article <371sh0$8qe at server.st.usm.edu>, gshearer at whale.st.usm.edu (Glen
> Just out of curiosity....why does everyone use MOPS
> buffer for RNA gels? Why not use autoclaved TBE or TAE?
Could it be because Tris has free amino groups that will form Schiff
adducts with formaldehyde whereas the morpholine core of MOPS is
non-reactive to this? Its been a long time since I did my organic chem
David S. Huen Phone: (0223) 333921
CRC Human Cancer Genetics Group Fax: (0223) 333875
Dept. of Pathology e-mail: smh1008 at cus.cam.ac.uk
University of Cambridge
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