Why MOPS for RNA gels?

David S. Huen smh1008 at cus.cam.ac.uk
Mon Oct 10 03:06:58 EST 1994

In article <371sh0$8qe at server.st.usm.edu>, gshearer at whale.st.usm.edu (Glen
Shearer) wrote:

> Just out of curiosity....why does everyone use MOPS
> buffer for RNA gels?  Why not use autoclaved TBE or TAE?
Could it be because Tris has free amino groups that will form Schiff
adducts with formaldehyde whereas the morpholine core of MOPS is
non-reactive to this? Its been a long time since I did my organic chem

David S. Huen                        Phone: (0223) 333921
CRC Human Cancer Genetics Group      Fax:   (0223) 333875
Dept. of Pathology                   e-mail: smh1008 at cus.cam.ac.uk
University of Cambridge
CB2 1QP                              
United Kingdom

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