Why MOPS for RNA gels?

David S. Huen smh1008 at cus.cam.ac.uk
Mon Oct 10 03:06:58 EST 1994


In article <371sh0$8qe at server.st.usm.edu>, gshearer at whale.st.usm.edu (Glen
Shearer) wrote:

> 
> Just out of curiosity....why does everyone use MOPS
> buffer for RNA gels?  Why not use autoclaved TBE or TAE?
> 
Could it be because Tris has free amino groups that will form Schiff
adducts with formaldehyde whereas the morpholine core of MOPS is
non-reactive to this? Its been a long time since I did my organic chem
though!!!!


-- 
David S. Huen                        Phone: (0223) 333921
CRC Human Cancer Genetics Group      Fax:   (0223) 333875
Dept. of Pathology                   e-mail: smh1008 at cus.cam.ac.uk
University of Cambridge
CB2 1QP                              
United Kingdom



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