cycseq question/YAC sequencing

Sasha Kraev bckraev at
Mon Oct 10 05:22:31 EST 1994

I have had success with cycle sequencing lambda minipreps, using 32P
labelled primers 22-25 bases long, two-step cycling ( 92 to 70 C and back )
and detection with the PhosphorImager screens for two days. The amount 
of template in this format can be as low as 1 fmol. I guess it should work 
with YACs as well provided you can have reasonably pure genomic DNA.

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