Tritium screens / protein gels

Berthe M. Willumsen bmw at biobase.aau.dk
Mon Oct 10 03:09:47 EST 1994


Did you try to blot the proteins onto membrane and expose that? We are
considering buying  an imager (if we get the money), and I would be very
interested to know if membrane-bound proteins labelled with tritium will
work on their screens.

Berthe

-- 
Berthe Willumsen
Dept. Molecular and Cellular Biology
Univ. Copenhagen
bmw at biobase.aau.dk


In article <1994Oct5.133237.8751 at mbcf>, heath at mbcf.stjude.org (Richard)
wrote:

> I posted a few days ago about the lack of sensitivity in Molecular Dynamics
> Tritium screens when trying to get an image of a tritium-labeled protein gel. 
> Well, it turns out that the comapany do not intent for theses screens to be
> used for this purpose.  *Any* water left in the gel will absord the beta
> emission from the tritium --> no image!  The screens were designed for TLC
> plates and the like.  While nobody at the company told me to try them for
> protein gels, it was not clear from any of their literature that this wouldn't
> work, so I tried it anyway!  
> 
> So, sorry to Molecular Dynamics for suggesting their screens were no good, and
> sorry to anybody who rushed out and brought them!
> 
> Richard Heath (No affiliation to Mol. Dynamics OR their competitors)
> St Jude Children's Research Hospital



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