Why MOPS for RNA gels?

Jim Astwood jdastw at ccmail.monsanto.com
Mon Oct 10 18:57:43 EST 1994

In article <371sh0$8qe at server.st.usm.edu>, gshearer at whale.st.usm.edu (Glen
Shearer) wrote:

> Just out of curiosity....why does everyone use MOPS
> buffer for RNA gels?  Why not use autoclaved TBE or TAE?
> Also, I recall a note in Biotechniques about leaving out
> the formaldehyde in the gel.  The idea was that the
> formamide/formaldehyde in the sample buffer denatured the RNA
> and it separated fine!  Anybody ever try this?
> Glen

I find that lowering the percentage of formaldehyde in the gel to around 3%
has no deleterious effects, and makes the gel considerably less nausiating.
 Some people add formaldehyde to the tank buffer too, but this is not
needed at all. 

James D. Astwoood          
Monsanto Company
700 Chesterfield Parkway N.
Mail Zone GG4K
St. Louis. MO
E-Mail: jdastw at monsanto.com

More information about the Methods mailing list