>>>Help about formal/agarose gel <<<
Randall J. Cohrs
COHRS-R at Oberon.HSC.Colorado.edu
Mon Oct 10 13:13:15 EST 1994
> Subject: >>>Help about formal/agarose gel <<<
> Hello everybody...
> Is it possible to observe RNAs with B-Ethidium after electrophoresis in
> formaldehyde/agarose gel?
we do not stain the formaldehyde/agarose gel after electrophoresis,
but instead we add 1 ul of 1 Mg/ml EtBr to the 20 ul denatured RNA
sample just prior to loading the gel. The RNA is stained well enough
to see the 18s & 28s rRNA as well as std size RNA markers. Also the
stained RNA is seen on the nytrocellulose or Zeta probe blots (using
hand held UV lamp).
rjc in denver
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