electroporation cuvettes

[szcooley at dale.ucdavis.edu]

Zalman L. Suldan (zsuldan at STUD.MED.CORNELL.EDU) wrote:
: >I just checked with Bio-Rad and they quote $135.00 + $15 S&H for a bag
: >of 50, 0.4cm cuvettes. Is this about average or are there 'generic'
: >brands of cuvettes that people have used to satisfaction

: We routinely reuse our cuvettes since they are so expensive. Be sure to
: wash them immediately (and well!!) with water and then store them in 70%
: EtOH. Before  using, wash again with water, then 100% EtOH, and then let
: them dry (I put them in a dessicator for a couple of minutes). As long as
: you make sure to have a negative control to control for how dirty the
: cuvettes may be, you should not have a problem (BTW, my negative control
: plates are almost always very sparse-- if not empty).

: Zal

: _________________________________________________________________
: Zal Suldan
: Tri-Institutional MD/PhD Program - Department of Cell Biology and Genetics
: Memorial Sloan Kettering Cancer Center / Cornell University Medical College
: Replies to: Z-Suldan at ski.mskcc.org    or   ZSuldan at Stud.Med.Cornell.edu

Treatment with 30% HNO3 for 1hr will ensure a negative response.


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