UV-crosslinking of DNA-protien complex

Frances Hannan Zoology flh at mole.bio.cam.ac.uk
Tue Oct 11 07:39:13 EST 1994

anon writes:

>In article <65887.lyc at ibms.sinica.edu.tw>, lyc at IBMS.SINICA.EDU.TW
>("L.Y.Chau") wrote:

>>  Dear netters:
>>  We have obtained a BromodU-labelled oligonucleotide and planned to do 
>>  UV-crosslinking experiments.  As we know that BromodU is light-sensitive.  
>>  Do we need to perform all the experiments in the dark including 
>>  oligonucleotides annealing, DNA purification, radioactive labelling and 
>>  protein binding reaction before UV-crosslinking?
>>  Jonghwei Pang
>   I worked in a lab where we used photoreactive nucleotde analogs, to
>analyze transcription reactions, and we did all our reactions in dark
>brown microfuge tubes, however I can't remember where we got them, and
>this enabled us to do our reactions in the light. We worried about long
>term exposure to light but not short term, and we were using bromo-U
>derivatives that were more sensitive to light!

We get dark brown tubes directly from Eppendorf. Order number 0030 120.91
Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at mole.bio.cam.ac.uk                               

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