Best strategy from peptide sequences to cDNA clone?

Steve McKinney oswald at ICHR.UWA.EDU.AU
Tue Oct 11 05:30:50 EST 1994


for species that are quite different in evolution from mice man etc, it 
may be useful to get a few genes and their translated aa seq for your 
organism e.g. c elegans, and check out the codon bias for that partic 
organism and then design your primers or whatever from that.
it can make a HUGE difference to library screenings

steve mckinney




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