Idaho Technology RAPD analysis
Anthony V. Furano
avf at helix.nih.gov
Tue Oct 11 19:06:38 EST 1994
In article <1994Oct9.141719.33131 at cobra.uni.edu>, palasj9579 at cobra.uni.edu
> We are in the process of looking at an Idaho Technology thermocycler
> 10 ul samples. We're wanting to use the thermal cycler to RAPD analysis. Has
> anyone had any experience in using this kind of thermal cycler for running
> RAPDs, and what kind of protocol is used for successful amplification?
> Jeff Palas
> University of Northern Iowa
> Cedar Falls, IA
I only have time for a very short note, but we only gave it a short try.
It worked BUT you need to use long ramp times, in other words you have to
allow time for mismatched primer/template hybrids to be extended.
If you need more info, e-mail me and I'll dig up my protocol.
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