Codon Usage and Expression in E.coli.

vaughan at vaughan at
Wed Oct 12 16:31:05 EST 1994

I am having problems with expressing foreign proteins in E.coli. It looks 
like I am suffering from premature termination of translation, probably
due to codons rarely found in E.coli proteins.
	Has anyone tried to express these rare tRNAs  from plasmids to
compensate for this problem? Are these bacterial tRNA genes available?
Any input appreciated.
	Kevin Vaughan
	vaughan at

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