6X myc cassette request
22693mgr at msu.edu
Wed Oct 12 17:28:19 EST 1994
We are trying to tag a plant membrane protein with C-myc (12 aa),
but we haven't been able to detect it with the 9E10 monoclonal
anti-myc antibody. Someone suggested to use a multiple myc tag (6-myc);
does anybody have comments about that?
Does anybody have a 6-myc cassette with a stop codon at the C terminus,
so that we can fuse it directly at the C-terminus of our protein?
Moreover, does anybody have a better suggestion about how to tag a membranal
protein (6 transmembrane domains) with a different epitope? I would greatly
appreciate any help. Thanks a lot!
MSU-DOE Plant Research Institute
Michigan State University
East Lansing, MI 48824-1312
Email: 22693mgr at msu.edu
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