Inverse PCR

Viraj Master vam2 at
Wed Oct 12 10:51:10 EST 1994

In article <37gioc$pak at>, chealy at (Dr. C.P.
Healy) wrote:

> I want to design an inverse PCR experiment to isolate the 5' regions of a number
> of transcripts which are differently spliced. I have a number of references to
> start from but would like to hear the experiences in others. The main things th   
> I am worried about are the efficiency of circularisation of the cDNA (how dueo you
> ensure blunt endedness and what are the optimum conditions for intramolecular
> ligation) and how do I ensure efficient amplification of  long cDNA fragments
> (e.g. 2-3kb - do I need to use one of the new Taq preparations?). Please reply
> by e-mail - I'll post if there's enough interest.

Chris, please do post a summary.



Viraj Master
Dept. of Organismal Biology and Anatomy
University of Chicago

More information about the Methods mailing list