Codon Usage and Expression in E. coli

[Cornelius Krasel]

Rebecca Schall (REBECCAS at PANVERA.COM) wrote:
: Does anyone know of a software program which would suggest 
: changes in codon usage (i.e., mutations to introduce) when trying to 
: express a mammalian gene in E. coli to overcome this "rare codon" 
: problem?

The GCG program "backtranslate" offers the option to choose a sequence
according to a certain codon usage table. You would start with a protein
sequence, backtranslate it and compare it with your original DNA
sequence.

Hope that helps,

--Cornelius.

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