Codon Usage and Expression in E. coli

Cornelius Krasel krasel at alf.biochem.mpg.de
Thu Oct 13 14:36:44 EST 1994


Rebecca Schall (REBECCAS at PANVERA.COM) wrote:
: Does anyone know of a software program which would suggest 
: changes in codon usage (i.e., mutations to introduce) when trying to 
: express a mammalian gene in E. coli to overcome this "rare codon" 
: problem?

The GCG program "backtranslate" offers the option to choose a sequence
according to a certain codon usage table. You would start with a protein
sequence, backtranslate it and compare it with your original DNA
sequence.

Hope that helps,

--Cornelius.

--
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de                 fax: +49 89 8578 3795 */
/* "People are DNA's way of making more DNA." (Edward O. Wilson, 1975)    */



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