Codon Usage and Expression in E. coli

Rebecca Schall REBECCAS at PANVERA.COM
Thu Oct 13 11:14:15 EST 1994


With regard to Kevin Vaughan's (vaughan at sci.wfeb.edu) recent 
posting concerning rare codon usage and the use of expressed tRNAs 
as compensation, I have an additional question.
Does anyone know of a software program which would suggest 
changes in codon usage (i.e., mutations to introduce) when trying to 
express a mammalian gene in E. coli to overcome this "rare codon" 
problem?
Input greatly appreciated.  Best regards,
Rebecca Schall
rebeccas at panvera.com



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