EXONUCLEASE III

[Klaus.Matthaei at ANU.EDU.AU]

>In Article <37h0se$6hd at mserv1.dl.ac.uk>, Michael Austin Roberts
><mar at st-andrews.ac.uk> wrote:
>>Dear Subscribers
>>
>>A perhaps niave question from a novice: will Exonuclease III cleave blunt-
>>ended double-stranded DNA at both ends?
>>Any advice would be appreciated.
>>
>>
>>Mike Roberts
>>
>>
>
>No, Exo III does not digest from blunt ends.
    ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>    Tracy

? since when?

We have been doing that for years.

Am I missing something.

Cheers, Klaus

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Klaus Matthaei
Head, Gene Targeting                            |           |
The John Curtin School of Medical Research      |  _--_|\   |
The Australian National University              | /      \  |
Snail mail: Canberra, ACT 0200, Australia       | \_.--._/<<|
E-mail: Klaus.Matthaei at anu.edu.au               |       v   |
Landline: 61 6 249 3782
                        "Sometimes I'm the Louisville slugger"
                        "Sometimes I'm the Ball"
                                Dire Straits
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