5'- 3'

Lauri Lintott llintott at ACS.UCALGARY.CA
Thu Oct 13 16:55:08 EST 1994


I need to chew up a piece of DNA 5' to 3' in a buffer that is pH
7.5.  I thought maybe I could use DNA pol 1.  I assume that if I
don't add any nucleotides the 5' to 3' exonuclease activity of
this enzyme should be favored.  Does this sound right to any one?
Has any one tried it?
Thanks in advance.
-- 
Lauri Lintott
Biological Siences, Universitiy of Calgary
llintott at acs.ucalgary.ca



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