5'- 3'

Lauri Lintott llintott at ACS.UCALGARY.CA
Thu Oct 13 16:55:08 EST 1994

I need to chew up a piece of DNA 5' to 3' in a buffer that is pH
7.5.  I thought maybe I could use DNA pol 1.  I assume that if I
don't add any nucleotides the 5' to 3' exonuclease activity of
this enzyme should be favored.  Does this sound right to any one?
Has any one tried it?
Thanks in advance.
Lauri Lintott
Biological Siences, Universitiy of Calgary
llintott at acs.ucalgary.ca

More information about the Methods mailing list