isolation of nuclei from lymphocytes

[michelle tourigny]

I was wondering if anyone out there has a more efficient way of isolating
nuclei (for DNase 1 digestion) from lymphocytes (thymus, lymph nodes and
spleen).  I have been using NP-40 overnight at 4 degrees C while the cells
are encased in low melting point agarose but I have gotten inconsisent
results between spleen and lymph node cells.  Any suggestions?

Thanks 
Michelle, grad student in progress

-- 
Michelle Tourigny
mrtour at stud.med.cornell.edu

Elmo says:  "But I'm not a scary monster"