Method for Touchdown PCR

Paiks paiks at aol.com
Fri Oct 14 23:16:05 EST 1994


In article <35nhm2$q5u at news.mic.ucla.edu>, Ron Kagan
<rkagan at ewald.mbi.ucla.edu> writes:
>An alternative approach to determine annealing temperatures may be
>offered by _Stratagene's_ , Robocycler which has a block capable of
>maintaining a temperature gradient, so it is possible to test out 8
>different annealing temperatures or so at once!
>Does anybody out there have a Robocycler and tried this?

I have Stratagene Robocylcer gradient type and tried it several times to
determine the optimum annealing temperature. It works as claimed. It is a
nice and fast machine.
Another method is to try optimizing the buffer system using Invitrogen's
PCR Optimizer kit, which I had great success with.



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