Codon Usage and Expression in E. coli

Eric Carstens Eric.B.Carstens at
Fri Oct 14 11:14:32 EST 1994

A plasmid expressing a ARG tRNA (UCU) was used in conjuction with an
expression plasmid in E.coli BL21 to successfully express a mammalian
gene which in the absence of the tRNA-expressing plasmid, was not over
expressed. However, the product was insoluble so we have not worked
further with it.The tRNA plasmid came from Dr. Ralf Mattes, Institute
for Genetics, Allmanmdring 31, D-70569, Stuttgart, Germany.

Posted for Peter Hloch, University of Konstanz by Eric Carstens

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