Sequencing with degenerate primers
cupton at sol.uvic.ca
Fri Oct 14 12:24:24 EST 1994
Does anyone have a reference to sequencing with Sequenase (ds DNA) using
degenerate primers? I was going to try the method used for multiple short
primers. Can't find the reference.....
Is it simply, anneal and extend+label on ice, then finish with ddNTPs at 37C?
It would save a bunch of $$.
Biochemistry & Microbiology
University of Victoria
PO Box 3055, Victoria
BC, Canada V8W 3P6
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