Sequencing with degenerate primers

Chris Upton cupton at
Fri Oct 14 12:24:24 EST 1994

  Does anyone have a reference to sequencing with Sequenase (ds DNA) using
degenerate primers? I was going to try the method used for multiple short
primers. Can't find the reference.....

Is it simply, anneal and extend+label on ice, then finish with ddNTPs at 37C?

It would save a bunch of $$.

 Chris Upton  
Biochemistry & Microbiology
University of Victoria
PO Box 3055, Victoria
BC, Canada   V8W 3P6

(604)721-8855 fax

More information about the Methods mailing list